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Preparation method for isochromophilone VIII and application of same in preparation of antineoplastic drugs

A technology of antineoplastic drugs and drugs, applied in the field of sochromophi

Inactive Publication Date: 2012-10-24
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, in the literature reports at home and abroad, there is no report that the compound isochromophilone VIII has anti-tumor activity

Method used

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  • Preparation method for isochromophilone VIII and application of same in preparation of antineoplastic drugs
  • Preparation method for isochromophilone VIII and application of same in preparation of antineoplastic drugs
  • Preparation method for isochromophilone VIII and application of same in preparation of antineoplastic drugs

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Embodiment 1

[0033] 1. Isolation, purification and identification of marine fungus Penicillium sp.FS60

[0034] The marine fungus Penicillium sp.FS60 of the present invention is isolated from sediments collected from the South China Sea (16°24.278′N, 112°10.390′E) at a depth of 955m, and identified by ITS sequence analysis. The base of the ITS region fragment The base sequence is shown in SEQ ID NO.1, and the GenBank gene accession number is: EU076956. After blast comparison and homology analysis, it was identified that the strain belongs to Penicillium sp. and named Penicillium sp.FS60. The bacterium was preserved in China Center for Type Culture Collection (CCTCC) on November 24, 2011, address: China. Wuhan. Wuhan University, and its preservation number is CCTCC NO: M2011413.

[0035] 2. Liquid fermentation of Penicillium sp.FS60

[0036]The medium is semi-seawater potato glucose liquid medium, and each liter of medium is prepared by the following method: boil 200g of potatoes with 500m...

Embodiment 2

[0046] The antitumor activity of compound isochromophilone VIII was tested by SRB method [Skehan P, Storeng R, Dominic S. New colorimetric cytotoxicity assay for anticancer-drug screening [J]. JNatl Cancer Inst,].

[0047] 1. Reagents for the test: the compound isochromophilone VIII prepared by the present invention was dissolved in dimethyl sulfoxide (DMSO) to obtain a mother solution with a concentration of 10 mg / mL, and then diluted to the required concentration with RPMI-1640 medium. The positive control was cisplatin aqueous solution.

[0048] The tumor cell lines used in this experiment were glioma cell SF-268, breast cancer cell MCF-7 and non-small cell lung cancer cell NCI-H460.

[0049] 2. Experimental method: Take NCI-H460, SF-268, and MCF-7 cells in the logarithmic growth phase, digest with trypsin, and count with trypan blue staining. After the trypan blue exclusion test detects that the cell viability is greater than 95%, use fresh RPMI-1640 medium adjusted cell ...

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Abstract

The invention discloses a preparation method for isochromophilone VIII and application of the same in preparation of antineoplastic drugs. According to the invention, isochromophilone VIII with antineoplastic activity is isolated from a liquid fermentation culture of the marine fungus Penicillium sp. FS60. According to results of experiments, it is found that the IC50 values of isochromophilone VIII in inhibiting SF-268 cells, MCF-7 cells and NCI-H460 cells are 7.17, 6.36 and 22.27 mu g / mL, respectively; and isochromophilone VIII has significant antineoplastic activity. The invention providesa candidate drug for research and development of novel antineoplastic drugs and scientific bases for development and utilization of marine microbial resources.

Description

Technical field: [0001] The invention belongs to the field of medical biotechnology, and specifically relates to a method for preparing isochromophilone VIII by using marine fungus Penicillium sp.FS60, and the application of the compound isochromophilone VIII in the preparation of antitumor drugs and as an active ingredient of antitumor drugs. Background technique: [0002] Cancer is one of the major diseases that endanger human health at present. According to data released by the International Union Against Cancer, in 2008, 12.7 million people worldwide suffered from cancer and 7.6 million died. Worldwide, cancer kills more people than AIDS, malaria, and tuberculosis combined. If effective measures are not taken, it is estimated that by 2030, there will be 26 million new cancer cases and 17 million cancer deaths every year. The incidence of cancer in my country is in a period of rapid increase, with about 2.6 million cancer cases and 1.8 million deaths each year. Cancer ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06A61K31/352A61P35/00C12R1/80
Inventor 陈玉婵张玲李冬利王磊章卫民
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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