Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof

A chloroacetamide and herbicide technology, which is applied in the field of environmental pollution bioremediation, can solve problems such as simultaneous degradation that cannot be seen, and achieves the effect of protecting health, reducing the phytotoxicity of herbicides to crops, and having a good removal effect.

Active Publication Date: 2012-10-31
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there have been reports on the microbial degradation of chlorinated acetamide herbicides at home and abroad, but so far there has been no simultane

Method used

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  • Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof
  • Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof
  • Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of strains

[0032] The enrichment matrix used to enrich the chloroacetamide herbicide-degrading strains was taken from the activated sludge in the pesticide wastewater biochemical treatment pond of the Lvlilai Pesticide Plant in Kunshan City, Jiangsu Province, and 5.0g of the activated sludge was added to 100ml of basic salt medium , Add 50mg·L -1 Butachlor, 30℃, 180r·min -1 Cultivate for 7 days, transfer 5% of the inoculum to the same medium, transfer 3 times in a row, dilute the enrichment solution stepwise, and take 10 -4 ~10 -7 Diluted enrichment solution 0.1mL each coated with 100mg·L -1 On a solid medium plate of butachlor, cultured at 30°C for 4 days, pick out the single colonies that grow and inoculate them with 100 mg·L -1 In the culture medium of various chloroacetamide herbicides (butachlor, acetochlor, alachlor and metolachlor), 30℃, 180r·min -1 Incubate on a shaker for 5 days to verify the degradation effect. The basic sa...

Embodiment 2

[0036] Example 2 Fermentation of repairing bacteria

[0037] The process of using the above-mentioned strain DC-2 to produce the repairing inoculum is: slant seed-shake flask seed liquid-seed tank-product (the packaging formulation is liquid inoculum).

[0038] 1) Inoculate the test tube of DC-2 (CCTCC NO: M 2012190) in the fermentation medium, and shake culture to the logarithmic phase;

[0039] 2) Inoculate the above-mentioned cultured strains at an inoculum of 10% (v / v, based on the volume of the culture medium) into a filling volume of 70% (v / v, based on the volume of the fermenter, the same below) 500 liters of seed tanks, cultivated to the logarithmic growth phase;

[0040] 3) The seed liquid is cultured in a production tank with a volume of 70% of the inoculum of 10% (v / v, based on the volume of the medium, the same below);

[0041] 4) During the cultivation process of the seed tank and the production tank, the aeration rate of sterile air is 1:1.0, the stirring speed is 220 rpm...

Embodiment 3

[0043] Example 3 Experiment on the biodegradation of chloroacetamide herbicides by DC-2 in the culture medium

[0044] Add alachlor, acetochlor and butachlor at a final concentration of 100mg / L to the basic salt medium (same as Example 1), and 1% of the inoculum is connected to DC-2 (CCTCC NO: M 2012190) bacteria Solution, set up inoculation inactivated DC-2 (CCTCC NO: M 2012190) control, culture in a 30 ℃ constant temperature incubator. Regular samples were taken to detect the degradation of alachlor, acetochlor and butachlor by the strains. The results are shown in Table 1. The degradation rate of strain DC-2 (CCTCC NO: M 2012190) to alachlor, acetochlor and butachlor reached 93.4%, 96.7% and 90.5% within 3 days, and to alachlor and acetochlor within 5 days The degradation rates of and butachlor reached 98.2%, 99.6% and 95.7%, respectively.

[0045] Table 1 The effect of strain DC-2 on alachlor, acetochlor and butachlor in the medium

[0046]

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Abstract

The invention belongs to the field of environmental contamination bioremediation, and discloses a chloro acetamide herbicide degrading bacteria, as well as a bactericide produced thereby and application thereof. Sphingobium quisquiliarum DC-2 is preserved in China Center for Type Culture Collection on 30 May, 2012, with the preservation number being CCTCC M2012190. The bactericide prepared by thebacterial strain can degrade multiple chloro acetamide herbicides such as alachlor, acetochlor and butachlor at the same time; by using the degrading bacteria, the residual quantities of alachlor, acetochlor and butachlor in soil and water environments can be reduced by more than 95% in a short time; in addition, the degrading bacteria can eliminate residues of alachlor, acetochlor and butachlor in agricultural products, and solves the problem about hazards to the soil and the water environments, the crops and human bodies by alachlor, acetochlor and butachlor.

Description

Technical field [0001] The invention belongs to the field of environmental pollution bioremediation, and relates to a chloroacetamide herbicide-degrading bacteria, and the produced bacteria and application thereof. Background technique [0002] Chloroacetamide herbicides are highly effective and highly selective herbicides, named after the chloroacetamide group in the molecular structure. At present, the main varieties include acetochlor, butachlor, alachlor and metolachlor, which are widely used in agriculture to control annual gramineous weeds and some broad-leaved weeds. Chloroamide herbicides are currently one of the most widely used herbicides in the world. At present, the annual output and area of ​​use are second only to organophosphorus herbicides, ranking second in the world, and with the reduction of agricultural labor force and agricultural cultivation methods Change, the demand and usage of this type of herbicide will continue to increase. [0003] Chloroacetamide her...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01A62D101/04A62D101/28C02F101/38
Inventor 何健李怡陈青李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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