Flavonoid metabolic profiling analysis method based on nanofluidic channel liquid chromatogram/mass spectrum
A technology of metabolic profiling and liquid chromatography, applied in the direction of analyzing materials, material separation, measuring devices, etc., to achieve the effect of ensuring stability, ensuring stability, and simple pretreatment process
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Embodiment 1
[0024] 1. Soybean Seed Sample Collection
[0025] The five soybean varieties are: Jiyu 73, Changnong 16, Jiunong 30, Hedou 12, and Zhonghuang 13. Among them, Jiyu 73, Changnong 16 and Jiunong 30 are planted in Jilin. Hedou 12 and Zhonghuang 13 were planted in Jinan. Soybean seed samples were stored in a -80°C refrigerator after collection.
[0026] 2. Analysis method
[0027] 2.1 Soybean seed sample pretreatment
[0028] Soybean seed samples were taken out from the -80°C freezer and waited in a desiccator for their temperature to return to room temperature. Each soybean seed sample was pulverized in a pulverizer and passed through a 60-mesh sieve to obtain a powder with uniform particle size. Divide soybean seed powder into plastic tubes and set aside.
[0029] Weigh 3 parts (0.1 g each) of soybean seed samples of 5 varieties and place them in 5 mL glass tubes respectively.
[0030] Weigh 0.5 g of each soybean seed sample, mix well, and use it as a quality control (QC) ...
Embodiment 2
[0051] In order to further illustrate the characteristics of the method described in this patent, the applicant compared different sample pretreatment methods and chips with different enrichment capacities.
[0052] 1) The soybean sample was extracted and purified by SPE method to improve the response of low-concentration compounds. The difference from Example 1 is that the Oasis HLBSPE column was used to pre-enrich and purify the soybean sample according to the standard procedure after the soybean sample was sonicated for 20 minutes. , the flow rate is about 10ml / min, and then washed with 1ml of water and 0.05ml of methanol, the target extract is finally washed with 0.5ml of methanol and 0.5ml of methanol / dichloromethane (1:1), and the collected solution is lyophilized and rinsed with 1ml of water Reconstitution, sample loading analysis, all analysis conditions and parameters are the same as in Example 1. It was found that after the second extraction, the response of most co...
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