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Compound type biological flocculating agent two-stage fermentation method

A biological flocculant and fermentation method technology, applied in the field of flocculant fermentation technology, can solve the problem of little research on bacteria

Inactive Publication Date: 2006-07-19
哈尔滨益生环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on cellulose-degrading bacteria at home and abroad focuses on the extraction of high-efficiency cellulase and its structural analysis, and enzyme production and enzymatic hydrolysis are carried out separately; it focuses on the screening of molds in cellulose-degrading bacteria. little

Method used

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specific Embodiment approach 1

[0004] Specific embodiment 1: This embodiment is achieved in the following way: a. Use cellulose biomass materials such as straw as raw materials, and use a combined physical-chemical method for pretreatment to improve the degradation rate of cellulose in the subsequent fermentation stage, Namely: ① First, the raw materials are mechanically crushed, ② then treated with de-wood chemical reagents, ③ steamed and exploded after treatment, to further break the crystal structure and protective layer of cellulose molecules, and make the material fluffy; b. Prepare the culture medium , wherein the raw material accounts for 1-5% of the mass-volume ratio of the culture solution, while adding inorganic salts thereto to meet the nutritional requirements of microbial fermentation, adjusting the pH value to 7.2-7.5 with NaOH and then sterilizing for subsequent use; c, the first The first-stage fermentation unit is the saccharification section of cellulose. Cellulose-degrading bacteria are ad...

specific Embodiment approach 2

[0005] Specific embodiment 2: This embodiment is achieved in the following way: a. Use straw cellulose biomass material as raw material, and use physical-chemical combination method for pretreatment to improve the degradation rate of cellulose in the subsequent fermentation stage, that is : ①first mechanically pulverize the raw materials, ②treat with dewooding chemical reagents, ③explode with steam after treatment, further break the crystal structure and protective layer of cellulose molecules, and make the material fluffy; b, prepare culture medium, Wherein the raw material accounts for 1% of the mass volume ratio of the culture solution, and inorganic salts are added thereto to meet the nutritional requirements of microbial fermentation, and the inorganic salts are composed of the following components: 1g / L KH 2 PO 4 , 0.3g / L MgSO 4 ·7H 2 O, 0.1g / L NaCl, 0.01g / L FeCl 3 , 2.5g / L NaNO 3 , use NaOH to adjust the pH value to 7.2-7.5 and then sterilize it for later use; c, th...

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Abstract

A two-stage fermenting process for preparing composite biologic flocculant includes such steps as physically and chemically pretreating cellulose, preparing culture liquid, adding inorganic salt, regulating pH value, sterilizing, adding cellulose gradating bacteria to convert cellulose to glucose and cellodiase, and adding flocculant generating bacteria.

Description

Technical field: [0001] The invention relates to a fermentation process of a flocculant. Background technique: [0002] In the treatment of urban water and industrial wastewater, the flocculation (coagulation) process is one of the most widely used key links, so the development of efficient and non-toxic flocculants has become an important content in the field of environmental science and engineering. It is worth noting that the research trend of today's flocculation process has gradually developed from focusing on process optimization in the past to focusing on the development of pharmaceuticals. Bioflocculant (Bioflocculant) is a kind of special polymer metabolite produced by microorganisms that can coagulate and precipitate non-degradable solid suspended particles, bacterial cells and colloidal particles in liquid. At present, the preparation of bio-flocculants mostly uses sugar or agricultural by-products as raw materials, and prepares bio-flocculants according to the i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/52
Inventor 马放任南琪朱艳彬杨基先远立江
Owner 哈尔滨益生环境技术有限公司
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