Construction method and application of complementary deoxyribonucleic acid (cDNA) of somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum

A coding gene and embryogenesis technology, applied in the field of construction of SERK coding gene cDNA, can solve the problem of embryoid bodies failing to develop into seedlings, and achieve the effects of fast reproduction speed, high plant survival rate, and short tissue cycle

Inactive Publication Date: 2012-11-14
BEIJING UNIV OF AGRI
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Problems solved by technology

[0004] In 1982, Geie T. firstly reported that the embryoid body was obtained by culturing

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  • Construction method and application of complementary deoxyribonucleic acid (cDNA) of somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum
  • Construction method and application of complementary deoxyribonucleic acid (cDNA) of somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum
  • Construction method and application of complementary deoxyribonucleic acid (cDNA) of somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum

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Embodiment Construction

[0070] The study of plant somatic embryogenesis is of great significance both in theory and in practice. In terms of theoretical exploration, plant somatic embryogenesis is an important manifestation of the totipotency of plant cells and a unique phenomenon in the process of plant asexual reproduction. The process of somatic embryogenesis is affected by genotype, hormones, stress, light, and mineral elements in the medium, Nitrogen source, carbon source and many other factors. But in the final analysis, somatic embryogenesis is that somatic cells start the expression of certain specific genes under the action of various internal and external factors, so that the differentiation and development of somatic cells are inhibited, and dedifferentiation and redifferentiation are transformed into embryogenic cells.

[0071] SERK (Somatic Embryogenesis Receptor-like Kinase) gene exists widely in monocotyledonous and dicotyledonous plants, and has specific expression patterns in differe...

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Abstract

The invention discloses a construction method and application of complementary deoxyribonucleic acid (cDNA) of a somatic embryogenesis receptor-like kinase (SERK) coding gene for regulating somatic embryogenesis of anthurium andraeanum. Initiation codon of a coding gene cDNA sequence is located at 19 base pairs, termination codon of the coding gene cDNA sequence is located at 1888 base pairs, and 622 amino acids are coded in total. The tender leaf of a mature anthurium andraeanum plant is taken as an explant, and through three stages of initiating culture, subculture and development culture, a regeneration plantlet is successfully obtained through an indirect somatic embryogenesis method; the induction callus period of the explant is shorter, and the plant has a high survival rate, quick propagation speed and stable heredity and is not easy to generate cytometaplasia; and the whole SERK gene cDNA sequence of the anthurium andraeanum is separated and cloned, and the SERK gene expression in various periods of different culture stages are analyzed at a transcriptional level to deepen the understanding on the somatic embryogenetic mechanism of the anthurium andraeanum, thereby disclosing the SERK coding gene cDNA for regulating the somatic embryogenesis of the anthurium andraeanum and the construction method of the SERK coding gene cDNA, and providing a high reference value for the function of SERK genes in somatic embryo sex transformation.

Description

technical field [0001] The invention relates to plant somatic embryogenesis, in particular to a construction method and application of a SERK coding gene cDNA regulating anthurium somatic embryogenesis. Background technique [0002] Anthurium andraeanum, also known as Anthurium and Anthurium, is a plant of the genus Anthurium in the family Araceae, native to the tropical rain forests of South America. Its unique flower shape, bright and gorgeous spathes, rich colors, long flowering period, unique leaf shape, and excellent foliage and flower viewing are rare decorative treasures for living rooms. In the world flower trade, Anthurium is second only to tropical flowers. Orchid is the second largest tropical flower. my country introduced cultivation in the 1970s, and it has become a rare cut flower variety in the market. [0003] Anthurines are usually propagated by division, which is very slow. Occasionally, cuttings are also used for propagation, but the survival rate is ver...

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IPC IPC(8): C12N15/54C12N15/10A01H5/00
Inventor 张克中崔金腾张睿鹂王爱香
Owner BEIJING UNIV OF AGRI
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