Method and device applicable to observing fish embryo development under stereo microscope

A stereoscopic microscope, embryonic development technology, applied in the fields of application, fish farming, animal husbandry, etc., to achieve the effect of simple and feasible observation and research

Inactive Publication Date: 2012-11-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention mainly aims at the problems existing in the current research on the development of fish embryos, and provides a method and device suitable for observing the development of fish embryos under a stereo microscope

Method used

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  • Method and device applicable to observing fish embryo development under stereo microscope
  • Method and device applicable to observing fish embryo development under stereo microscope
  • Method and device applicable to observing fish embryo development under stereo microscope

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Embodiment Construction

[0022] Below in conjunction with embodiment, the present invention will be further described.

[0023] Such as figure 1 As shown, the device is composed of a high-level water storage device 1, a low-level water storage device 7, an incubator 9, and a waste liquid container 11. The high-level water storage device 1 communicates with the low-level water storage device 7 and the incubator 9 in sequence according to the height difference. The waste liquid container 11 is installed under the side of the incubator 9; the high-level water reservoir 1 communicates with the low-level water reservoir 7 through the first communication pipe 3; a flow rate control valve 4 is installed in the middle of the first communication pipe 3 ; Heating rod 5 and oxygenation pump 6 are also housed in the described low-level water storage 7; The described low-level water storage 7 is connected with the incubator 9 through the second connecting pipe 8, and an outlet pipe 10 is equipped on one side there...

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Abstract

The invention belongs to a method and a device applicable to observing fish embryo development under a stereo microscope. The device is characterized by comprising a high-level water container, a low-level water container, a hatcher and a waste liquid container, wherein the high-level water container is sequentially communicated with the low-level water container and the hatcher according to high potential difference, the waste liquid container is arranged at the side lower part of the hatcher; the high-level water container is communicated with the low-level water container by virtue of a first communicating pipe; the first communicating pipe is provided with a flow rate control valve in the middle; the low-level water container is also internally provided with a heating rod and an aeration pump; and the low-level water container is connected with the hatcher by virtue of a second communicating pipe, one side of the low-level water container is provided with a water outlet pipe, and waste liquid is discharged into the waste liquid container. The hatcher of the device disclosed by the invention also can be manufactured by adopting glass, organic glass or transparent plastic. The invention also discloses the method for observing fish embryo development under the stereo microscope by utilizing the device disclosed by the invention.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method and a device suitable for observing the development of fish embryos under a stereo microscope. Background technique [0002] Fish embryonic development is an important stage in the early developmental history of fish, and its development process is: fertilized egg→cleavage stage→blastocyst stage→gastrula stage→organogenesis stage→membranous hatching. During this process, fertilized eggs will experience the most intense development and differentiation process in the entire life history. This period is relatively fragile and has a high mortality rate. Hatch rate and so on have an important guiding role. [0003] At present, the research on the development of fish embryos is generally carried out in the laboratory. The fertilized eggs of fish are placed in a container, slightly inflated, and the water temperature in the container is controlled by heating ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K63/04A01K63/06
Inventor 祝东梅宋文杨坤张新辉温久福王卫民
Owner HUAZHONG AGRI UNIV
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