Fusion protein of Exendin-4 and mutational human serum albumin, and preparation method of fusion protein

A human serum albumin and fusion protein technology, applied in the field of long-acting recombinant fusion protein drugs, can solve the problems of small molecular weight, large changes in serum concentration, and inconvenience to patients, and achieve good application prospects and prolong the effect of action time
CN102827286AInactive Publication Date: 2012-12-19SHANGHAI ALLIST PHARM CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI ALLIST PHARM CO LTD
Publication Date
2012-12-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a fusion protein of Exendin-4 and mutational human serum albumin and a preparation method of the fusion protein, belonging to the technical field of long-acting recombined fusion protein drugs. The invention relates to the fusion protein of Exendin-4 and human serum albumin (HSA) in which the 410-site is mutated to A (Ala alanine) from R (Arg arginine). The fusion protein comprises two polypeptide zones, wherein zone I is composed of two Exendin-4 repetitive sequences, and zone II is site-mutation human serum albumin (HSA); the zone I is directly connected with the N-terminal of the zone II by the C-terminal of the zone I without adding any connecting peptides therebetween; and the structure of the fusion protein is: (Exendin-4)2-HSA (R410A). Compared with Exendin-4 and (Exendin-4)2-HAS, (Exendin-4)2-HSA (R410A) fusion protein provided by the invention has the characteristic of longer-lasting effect in vivo, and can be used for preparing long-lasting preparation of non-insulin-dependent diabetes.
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Description

technical field

[0001] A fusion protein of insulin secretion-stimulating peptide and mutated human serum albumin and a preparation method thereof belong to the technical field of long-acting recombinant fusion protein drugs. Background technique

[0002] Insulin-stimulating peptide (Exendin-4) is a peptide hormone that stimulates insulin secretion. It contains 39 amino acids. It was discovered in the early years from the saliva of the Gila monster that grew in the southwestern United States and the deserts of Mexico. 53% of its amino acid sequence is homologous to the human blood glucose regulating hormone, glucagon-like peptide-1 (GLP-1). The substance has the effect of directly lowering blood sugar and stimulating the secretion of insulin by the pancreas, and is a potential agonist of the GLP-1 receptor (GLP-1R); it can also slow down the absorption of food by slowing down the peristalsis of the gastrointestinal tract, thereby reducing The peak of glucose in the blood. A...

Claims

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