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Cultivation method of rice engineering maintainer line and application thereof to breeding of rice genic male sterile line

A technology of common sterile line and engineering maintenance line, applied in the field of reproduction of crop sterile lines, can solve the problems of costing a lot of manpower and material resources, and the number of common sterile lines is scarce, and achieves a great increase in probability, a wide recovery spectrum, and fertility. Sexually stable effect

Active Publication Date: 2014-03-05
HUNAN HYBRID RICE RES CENT
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Problems solved by technology

Through the above method, scientific researchers can breed a small amount of common CMS lines for scientific research and experiments, but the number of common CMS lines obtained by breeding is rare, and it takes a lot of manpower and material resources
If the problem of large-scale reproduction of common GMS lines can be solved, the large-scale application of common GMS lines to hybrid seed production will greatly release the utilization potential of heterosis

Method used

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  • Cultivation method of rice engineering maintainer line and application thereof to breeding of rice genic male sterile line
  • Cultivation method of rice engineering maintainer line and application thereof to breeding of rice genic male sterile line
  • Cultivation method of rice engineering maintainer line and application thereof to breeding of rice genic male sterile line

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Embodiment

[0032] rice TDR The protein encoded by the (Tapetum Degradation Retardation) gene controls the degradation of rice tapetum and is an important factor in the regulation network of rice tapetum development and degradation. if TDR When the gene is mutated, the velvet layer of rice anthers will degenerate, leading to abortion of rice pollen, and a common nuclear male sterile line of rice will be produced (see Chinese Patent Document No. CN1884541A).

[0033] A kind of application of the engineering maintainer line of the common GMS line of rice of the present invention in the propagation of the common GMS line of rice, the specific object of its application is the above-mentioned present embodiment tdr The general nuclear sterile line of the gene mutant specifically includes the following steps (the process flow can be found in figure 1 ):

[0034] 1. 60 Co-γ-ray mutagenesis obtained a rice sterile mutant. According to genetic analysis, it was shown that the sterility tra...

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Abstract

The invention discloses a cultivation method of a rice engineering maintainer line and a method for applying the cultivated rice engineering maintainer line to breeding of a rice genic male sterile line. The cultivation method comprises the following steps: firstly, positioning and cloning a male sterile gene s of the rice genic male sterile line and a fertile gene S of a male sterile line source parent through a map position cloning method; connecting a color marker gene C and the fertile gene S to the same dual expression vector to enable the color marker gene C and the fertile gene S to be subjected to linkage inheritance and coseparation after being introduced into a plant; transferring the expression vector in the genic male sterile line by a trangenosis method to obtain a fertility restorer strain; and then carrying out back-crossing on the fertility restorer strain and the genic male sterile line to obtain the engineering maintainer line. The cultivation method can be applied to breeding of the rice genic male sterile line, and can simplify the breeding process of the genic male line greatly.

Description

technical field [0001] The invention relates to the technical field of reproduction of crop sterile lines, in particular to a method for breeding male sterile lines of self-pollination crops. Background technique [0002] Using the male sterility of plants to breed male sterile lines, and then use this genetic tool to produce hybrid seeds in large quantities, can make the heterosis of many crops, especially self-pollination crops, be used in production. [0003] According to the three-type theory, male sterility in plants is divided into three genetic types: cytoplasmic male sterility, nuclear male sterility and cytoplasmic male sterility. [0004] Cytoplasmic male sterility means that the sterility traits are only controlled by cytoplasmic genes and have nothing to do with the nucleus. The sterility traits purely controlled by cytoplasmic genes have not been applied in production because there is no restorer line. [0005] Nuclear male sterility means that the sterility tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82
Inventor 袁定阳李新奇李莉段美娟余东邝翡婷宋书锋李娜谭炎宁孙志忠袁光杰袁贵龙符习勤赵炳然张大兵袁隆平
Owner HUNAN HYBRID RICE RES CENT
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