Universal type breeding method for rice engineering maintainer line, and application thereof in propagation of ordinary nucleic male sterility lines of rice

Abstract

The invention discloses a universal type breeding method for a rice engineering maintainer line. The method comprises the following steps: establishing a dual-element expression vector of color marker gene C; shifting the vector into a type with the genetype of SS through a transgenic method to obtain transgenic strains; obtaining isozygoty strains through selfing propagation; analyzing the insertion site of gene C of each isozygoty strain and obtaining an engineering maintainer line candidate bank through screening and establishment; and crossbreeding ordinary nucleic male sterility lines of rice with the genetype of SS with the isozygoty strains in the candidate bank to obtain F1, obtaining F2 through selfing propagation of F1, breeding seeds with no color in the F2, inspecting sterile stains in each group of F2 stain after breeding, and screening the seeds of F1, where the sterile stains accounting for more than 98% of the F2 come from to take the seeds as the engineering maintainer line. The breeding method provided by the invention has the advantages of short period, strong universality, high efficiency and the like; and the obtained engineering maintainer line can be applied to mechanized and large-scale propagation of ordinary nucleic male sterility lines of rice.

Description

technical field [0001] The invention belongs to the technical field of reproduction of crop sterile lines, in particular to a method for the propagation of male sterile lines of self-pollination crops (such as rice, wheat, etc.). Background technique [0002] Using the male sterility of plants to breed male sterile lines, and then use this genetic tool to produce hybrid seeds in large quantities, can make the heterosis of many crops, especially self-pollination crops, be used in production. [0003] According to the three-type theory, male sterility in plants is divided into three genetic types: cytoplasmic male sterility, nuclear male sterility and cytoplasmic male sterility. [0004] Cytoplasmic male sterility means that the sterility traits are only controlled by cytoplasmic genes and have nothing to do with the nucleus. The sterility traits purely controlled by cytoplasmic genes have not been applied in production because there is no restorer line. [0005] Nuclear male...

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Problems solved by technology

[0011] As more and more research institutions and scientific researchers realize the huge utilization value and potential of common GMS lines, many research institutions have recently increased their research investment and research on the large-scale reproduction of common GMS lines. efforts, and has made gratifying progress. For example, the Hunan Hybrid Rice Research Center has achieved success by locating and cloning the sterile gene s and the fertile gene S to construct an engineering maintainer line to propagate the common GMS line, but this method requires cloning The sterile gene s and the fertile gene S increase the difficulty of the construction of the engineering maintainer line and greatly prolong the progress of the construction of the engineering maintainer line

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