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Enzyme sensor for organophosphorus pesticide detection and preparation method thereof

An organophosphorus pesticide and enzyme sensor technology, applied in the field of enzyme chemical sensors, can solve the problems of expensive equipment, long analysis period, and complicated processing methods.

Inactive Publication Date: 2015-04-22
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can detect pesticide residues more accurately, there are disadvantages such as expensive equipment, cumbersome sample pretreatment methods, and long analysis cycle.

Method used

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  • Enzyme sensor for organophosphorus pesticide detection and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 3 mg of SiO with an average diameter of 30 nm 2 The powder was ultrasonically dispersed in 35 mL of twice distilled water, and 1.88 mL of 0.1 mol / L sodium citrate solution was added and stirred for 10 min. Slowly add 0.07 mL of hydroxylammonium hydrochloride (NH 2 OH·HCl) (0.1 mol / L) and 0.125 mL chloroauric acid (HAuCl 4 ) (1%), stirred for 20 hours to obtain gold-doped SiO 2 (Au-SiO 2 ) dispersion solution of composite nanoparticles. The particle size and distribution state of the prepared composite nanoparticles were investigated by scanning electron microscopy. As shown in the figure, the Au nanoparticles were uniformly doped in SiO 2 In nanoparticles, the average particle size is around 10 nm.

[0026] The bare glassy carbon electrode was covered with 0.5 μm diameter Al 2 o 3 The paste is ground and polished on the suede to make it into a mirror surface, and then in acetone and HNO with a volume ratio of 1:1 3 , NaOH (1mol / L) and ultrasonic treatment i...

Embodiment 2

[0028] Take 5 mg of SiO with an average diameter of 40 nm 2 The powder was ultrasonically dispersed in 30 mL double distilled water, 2 mL 0.3 mol / L sodium citrate solution was added, and stirred for 20 min. Slowly add 0.15 mL of hydroxylammonium hydrochloride (NH 2 OH·HCl) (0.3 mol / L) and 0.3 mL chloroauric acid (HAuCl 4 ) (3%), stirred for 24 hours to obtain gold-doped SiO 2 (Au-SiO 2 ) dispersion solution of composite nanoparticles.

[0029] The bare glassy carbon electrode was covered with 0.5 μm diameter Al 2 o 3 The paste is ground and polished on the suede to make it into a mirror surface, and then in acetone and HNO with a volume ratio of 1:1 3 , NaOH (1mol / L) and ultrasonic treatment in secondary water. Before modification, the glassy carbon electrode was placed in 0.5 mol / L H 2 SO 4 Scan until a stable cyclic voltammogram is obtained. Take 7 μL of Au-SiO 2 The nanoparticle dispersion solution was drop-coated on the surface of the dry bare glassy carbon elec...

Embodiment 3

[0031] Take 3 mg of SiO with an average diameter of 100 nm 2 The powder was ultrasonically dispersed in 35 mL of twice distilled water, and 1.88 mL of 0.5 mol / L sodium citrate solution was added and stirred for 10 min. Slowly add 0.8 mL of hydroxylammonium hydrochloride (NH 2OH·HCl) (0.1 mol / L) and 0.5 mL of chloroauric acid (HAuCl 4 ) (4%), stirred for 30 hours to obtain gold-doped SiO 2 (Au-SiO 2 ) dispersion solution of composite nanoparticles.

[0032] The bare glassy carbon electrode was covered with 0.5 μm diameter Al 2 o 3 The paste is ground and polished on the suede to make it into a mirror surface, and then in acetone and HNO with a volume ratio of 1:1 3 , NaOH (1mol / L) and ultrasonic treatment in secondary water. Before modification, the glassy carbon electrode was placed in 0.5 mol / L H 2 SO 4 Scan until a stable cyclic voltammogram is obtained. Take 15 μL Au-SiO 2 The nanoparticle dispersion solution was drop-coated on the surface of the dry bare glassy ...

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Abstract

The invention discloses an enzyme sensor for organophosphorus pesticide detection. The enzyme sensor comprises a bio-enzyme layer, a nano-particle layer and an electrode base, wherein a bio-enzyme solution is drip-coated on the nano-particle layer and a nano-particle solution is drip-coated on the electrode base. Nano-particles are Au-SiO2 nano-particles. The electrode base is a glassy carbon-made electrode base. The bio-enzyme solution comprises acetylcholinesterase and a chitosan acetate solution. The invention also discloses a preparation method of the enzyme sensor for organophosphorus pesticide detection. The enzyme sensor has an existing organophosphorus pesticide detection linear range of 6.0*10<-13> to 2.0*10<-10>mol / L, and an existing organophosphorus pesticide detection limit of 3.0*10<-13>mol / L.

Description

technical field [0001] The invention relates to an enzyme chemical sensor, in particular to an enzyme sensor for detecting organophosphorus pesticides and a preparation method thereof. Background technique [0002] Organophosphorus pesticides mainly refer to phosphate esters, thiophosphate esters, and phosphoramidate organophosphorus compounds. Because of their high insecticidal efficiency, low phytotoxicity to plants, and low persistence in the environment, they have become the most commonly used pesticides in my country. A class of pesticides widely used in various crops, especially fruits and vegetables. However, organophosphorus pesticides, which have been used in large quantities for a long time, remain in the environment and cause serious pollution to water bodies and soil. - Metabolism disorder of acetylcholine, causing various acute and chronic poisoning conditions, such as movement disorders, coma, paralysis and even death. Therefore, timely, accurate and sensitive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
Inventor 王丹张鹏王瑾何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH