Liquid chromatography-mass spectrum detection method for lutein disuccinate

A technology of succinate and lutein, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of good reproducibility, good precision and high sensitivity

Active Publication Date: 2014-09-10
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the qualitative and quantitative analysis of lutein disuccinate by HPLC-MS

Method used

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  • Liquid chromatography-mass spectrum detection method for lutein disuccinate
  • Liquid chromatography-mass spectrum detection method for lutein disuccinate
  • Liquid chromatography-mass spectrum detection method for lutein disuccinate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. Experimental instruments and reagents

[0027] 1) Experimental equipment

[0028] Agilent1290Infinity HPLC-6460MS coupled instrument, Agilent1200HPLC chromatograph (Agilent, USA), YMC-C30 column (YMC, Japan)

[0029] Purifier with Frac-900 (Sweden Amersham Co., Ltd.)

[0030] 85-2A double digital display constant temperature magnetic stirrer (Jiangsu Jintan City Ronghua Instrument Manufacturing Co., Ltd.)

[0031] D10 nitrogen purging instrument (Hangzhou Aosheng Instrument Co., Ltd.)

[0032] GF254 silica gel plate (Qingdao Ocean Chemical Factory)

[0033] 2) Experimental reagents

[0034] Standard lutein (≥96.9%, Chromadex, USA)

[0035] Chromatographically pure methanol, acetonitrile, dichloromethane (American Tiandi Company)

[0036] Analytical pure dichloromethane, acetonitrile, n-hexane, acetone, N, N-diisopropylethylamine (Sinopharm Chemical Reagent Co., Ltd.)

[0037] Analytical pure succinic anhydride (Chengdu Kelong Chemical Reagent Co., Ltd.)

[...

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Abstract

The present invention discloses an accurate and simple lutein disuccinate detection method. HPLC conditions comprise that: a YMC Carotenoid C30 chromatographic column is adopted, a solution comprising a 0.05% acetic acid methanol solution and a 0.05% acetic acid acetonitrile solution according to a volume ratio of 50:50 is adopted as a mobile phase, a time is 30 min, a flow rate is 1.0 mL / min, a DAD detector is adopted, a detection wavelength is 450 nm, a column temperature is 25 DEG C, and an injection volume is 20 muL. With the method, lutein and lutein disuccinate can be separated well. MS conditions comprise that: an APCI+ / MS positive ion mass spectrum is adopted, a flow rate of a chromatographic column effluent component entering a mass spectrometer is 10 muL / min, a scan range is m / z50-1200, a vaporization temperature is 350 DEG C, and a drying gas flow rate is 4 L / min. According to the present invention, qualitative lutein disuccinate analysis is performed based on a sample retention time, MS information and spectral information, and quantitative analysis is performed based on the peak area. The method has advantages of rapidness, efficiency, good reproducibility, high recovery rate, and the like.

Description

1. Technical field [0001] The invention relates to a method for measuring lutein disuccinate, which can be used for monitoring the synthesis reaction process of lutein and succinic acid and detecting reaction products. 2. Background technology [0002] Lutein (lutein) is a carotenoid containing hydroxyl, which has antioxidant effect, can prevent age-related macular degeneration, and delay the early process of arteriosclerosis. Phyllis E.Bowen et al. added the same dose of lutein ester and lutein to the diet of healthy subjects, and used the concentration of lutein in plasma × time (AUC) as the index to measure the bioavailability. The results showed that lutein The AUC of lutein esters is 61.6% greater than that of lutein. Succinic acid is an endogenous neuroactive regulating substance, which has anticonvulsant, antiepileptic, sedative, cooling and analgesic effects. Succinic acid is an intermediate metabolite of the tricarboxylic acid cycle, located in the pivotal positio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/72G01N30/02
Inventor 李大婧徐爱琴刘春泉肖亚东庞慧丽
Owner JIANGSU ACAD OF AGRI SCI
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