Trichoderma asperellum strain and application thereof

A technology of Trichoderma aculeatus and strains, applied in the fields of application, fungicides, fungi, etc., can solve the problems of unfavorable large-scale popularization and application, cumbersome preparation process, etc., achieve good biocontrol potential, prevent and control pepper blight, and strong parasitism Effect

Active Publication Date: 2013-02-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the complex biological preparation requires a variety of strains and raw materia...

Method used

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  • Trichoderma asperellum strain and application thereof
  • Trichoderma asperellum strain and application thereof
  • Trichoderma asperellum strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Isolation, purification, identification and preservation of the bacterial strain of embodiment 1

[0026] 1. Isolation and purification of Trichoderma

[0027] After fully mixing the soil collected from the capsicum rhizome soil of the disease, a small amount of soil particles were removed and placed on a PDA plate containing chloramphenicol, with 4 soil particles on each plate, and repeated three times. Incubate in the dark at 25°C. After Trichoderma spores, pick them out on a new PDA plate in time.

[0028] The strains for short-term storage are placed in PDA inclined test tubes and stored in a refrigerator at 4°C. Long-term storage of Trichoderma in a -20°C refrigerator (spores + 17% skimmed milk powder + silica gel particles).

[0029] 2. Identification of Trichoderma species

[0030] The biological characteristics of this strain are: the colony is cultured on PDA solid medium at 25°C, and it expands to 9cm in 3-4 days. The initial stage is white and sparse, and...

Embodiment 2

[0052] Embodiment 2 confrontation culture method

[0053] Trichoderma aculeatus Thz01 and Phytophthora capsici strains were transferred to PDA medium for activation. After Phytophthora capsici grew for 48 hours, a 0.5 cm diameter punch was used to cut the fungus cake at the edge of the colony, and the fungus cake was inoculated into the culture dish. At one end, after culturing at 25°C for 48 hours, inoculate the well-drilled Trichoderma strains to the other end of the petri dish, and treat each Trichoderma strain three times. At the same time, use only Phytophthora as a blank control, and observe whether there is an antagonistic effect after culturing for 7 days. And take pictures and record.

[0054] The results of confrontation culture experiment between Trichoderma aculeatus Thz01 strain and Phytophthora capsici strain are shown in figure 1 . No. 1 is the control, the plate on the left of plates 2, 3, and 4 is Phytophthora capsici, and the plate on the right is Trichoder...

Embodiment 3

[0055] Embodiment 3 prevents and treats capsicum blight

[0056] Preparation of biocontrol agent: Trichoderma aculeatus Thz01 was used to make biocontrol agent by using feed wheat grains as a substrate. The specific steps are:

[0057] (1) Strain activation: Trichoderma aculeatus Thz01 was inoculated into PDA medium and cultured at 25°C for 5 days;

[0058] (2) Preparation of seed liquid: after the activation of the bacterial strain, the spores were scraped off and scraped into sterilized water to prepare a live bacteria concentration of 10 7 The spore suspension of each / mL is the seed liquid;

[0059] (3) Inoculate the seed liquid into boiled and sterilized wheat grains, cultivate them for 15 days, and turn them in time during the culture period, so that the wheat grains can be fully grown with Trichoderma aculeatus Thz01 spores to prepare biocontrol agents; The dosage of seed solution is 3mL based on kilogram of wheat grains.

[0060] The prepared biocontrol preparation ...

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Abstract

The invention discloses a trichoderma asperellum strain and application thereof. The trichoderma asperellum strain is named as trichoderma asperellum Thz01, and the preservation number of the trichoderma asperellum strain is CGMCC (China general microbiological culture collection center) No.6422. The trichoderma asperellum strain is separated from a capsicum habitat, has a remarkable antagonism effect to phytophthora capsici, can be obviously subjected to hyperparasitism on the phytophthora capsici, an can effectively degrade mycelia of the phytophthora capsici, and a biocontrol agent prepared from the strain can be used for effectively preventing and treating phytophthora capsici leonian .

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a Trichoderma aculeatus strain and application thereof. Background technique [0002] Pepper blight, commonly known as "dead seedling disease", is a soil-borne disease caused by Phytophthora capsici. The main component of the cell wall of this pathogen is cellulose, which is different from the main component of the cell wall of common fungal pathogens (such as Ascomycetes and Basidiomycetes) which is chitin. Pepper blight is a devastating disease of pepper in my country, which can cause serious yield loss. Since the disease is a soil-borne disease, the pathogenic bacteria can survive in the soil for a long time, and this disease is also the most difficult to control in production. In particular, the biological characteristics of the pathogen are unique, and the methods and techniques of disease prevention and control are also obviously different from those of...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 张敬泽蒋恒萧芳
Owner ZHEJIANG UNIV
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