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Preparation of protein and polypeptide through low-temperature extrusion enzymolysis method

A protein and low-temperature technology, applied in fermentation and other directions, can solve the problems of low protein extraction rate, long time, low conversion rate of peptides, etc.

Active Publication Date: 2014-11-05
申德超
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is used to solve the problems of long process time, low protein extraction rate, low conversion rate of protein into polypeptide and high production cost in the preparation of proteins and polypeptides using existing technologies

Method used

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  • Preparation of protein and polypeptide through low-temperature extrusion enzymolysis method

Examples

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Effect test

Embodiment 1

[0017] Example 1 Preparation of corn polypeptide

[0018] A low-temperature extrusion enzymolysis method for raw materials with added enzyme preparations for preparing corn polypeptides, wherein the moisture content of corn gluten powder before extrusion is less than 14%, its particle size should pass through an 80-mesh sieve, and then an appropriate amount of water is added to make the moisture content The rate is adjusted to 40%, and an appropriate amount of neutral protease is added at the same time. The amount of addition is 5L or 5kg of neutral protease per ton of raw materials for preparing corn polypeptides before extrusion, and the pH value of the added water is adjusted to 6.8-8.0. The extrusion device of the single-screw extruder extrudes and processes the above-mentioned corn gluten powder with the moisture content adjusted to 40% for the preparation of corn polypeptides with enzyme preparations, wherein the extrusion system parameter range is: connecting the templat...

Embodiment 2

[0020] Example 2 Preparation of soybean protein isolate powder

[0021] A low-temperature extrusion enzymolysis method of defatted soybean meal added with an enzyme preparation for preparing soybean protein isolate powder, wherein the moisture content of the defatted soybean meal soaked in oil before extrusion is less than 14%, and its particle size should pass through an 80-mesh sieve , then add an appropriate amount of water to adjust the water content to 40%, and at the same time add an appropriate amount of alkaline protease, the amount added is 5L or 5kg of alkaline protease per ton of defatted soybean meal, adjust the pH value of the added water to 8-12, use The extrusion device of a commercially available single-screw extruder extrudes and processes the above-mentioned defatted soybean meal with the moisture content adjusted to 40% to prepare the soybean protein isolate and add an enzyme preparation, wherein the extrusion system parameter range is: connecting the templat...

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Abstract

The invention belongs to the technical field of preparation of a protein and a polypeptide through extrudates prepared by a low-temperature extrusion enzymolysis method in a process of preparation of the protein and the polypeptide. The main technical features are as follows: a low-temperature extrusion enzymolysis method of raw materials of an additive enzyme preparation for preparing the protein and the polypeptide, and a method of preparing a powdery protein or polypeptide product through carrying out conventional digestion, separation (single enzyme and multienzyme synergistic hydrolysis or alkali dissolution and acid deposition), purification, ultrafiltration, concentration, spray drying or freeze drying on crushing matters obtained after low-temperature drying of extrudates of the raw materials of the additive enzyme preparation for preparing the protein and the polypeptide. According to the invention, the time required for a technical process for preparing the protein and the polypeptide is 3 / 5-3 / 4 of the time required for the conventional technical process, the protein extraction ratio is more than 80%, the conversion rate for converting the protein to the polypeptide is more than 80%, and not less than 75% of a peptide section of the polypeptide product has a molecular weight of less than 1000 Da.

Description

technical field [0001] The invention belongs to the technical field of preparing proteins and polypeptides from extrudates processed by low-temperature extrusion and enzymolysis in the process of preparing proteins and polypeptides. Background technique [0002] The current process of preparing proteins and peptides is usually: preparation of raw materials for proteins and peptides → pretreatment (including: crushing, ultra-fine grinding, microwave treatment, ultrasonic treatment, extrusion puffing treatment (note: the extruded materials are not extruded before extrusion) Add any chemical reagents and enzyme preparations), etc.) → separation (single enzyme and multi-enzyme synergistic hydrolysis or alkaline extraction and acid precipitation) → purification → ultrafiltration → concentration → spray drying → making powdered protein or polypeptide products (Shi Yanguo, Ren Li .Technology of Soybean Products[M]. Beijing: China Light Industry Press. 1998.3; 56-60; Liu Jing, Zhan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 申德超申勋宇
Owner 申德超
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