Pseudomonas fluorescens capable of degrading estrogen substances and preparation method and application thereof

Abstract

Pseudomonas fluorescens capable of degrading estrogen substances is named as SJTE-2, and a preservation number is CGMCC No.6587. The pseudomonas fluorescens can use natural estrogen or environmental estrogen as the only carbon source to grow and can degrade estrogen substance oestrone, 17-Beta-estradiol, estriol and polycyclic aromatic hydrocarbon substance naphthalene, luxuriant, bisphenol A, and can be used for decomposing and removing an estrogen substance and a polycyclic aromatic hydrocarbon substance in an environment. Under the culture condition that the estrogen or the polycyclic aromatic hydrocarbon is used as the only carbon source, a bacterial strain SJTE-2 can degrade oestrone, 17-beta-estradiol and estriol within 24 hours, wherein the initial concentration of the oestrone, 17-beta-estradiol and estriol is 1 mg/L, and degradation rate of naphthalene, luxuriant and bisphenol A is over 90%, wherein the concentration of the naphthalene, luxuriant and bisphenol A is 50 mg/L. The bacterial strain can degrade oestrone, 17-beta-estradiol and estriol for more than 90% in 7 days, wherein the initial concentration of oestrone, 17-beta-estradiol and estriol is 50 mg/L, and degradation rate of naphthalene, luxuriant and bisphenol A is larger than 80%, wherein the concentration of naphthalene, luxuriant and bisphenol A is 500 mg/L.

Description

technical field [0001] The invention relates to genetic engineering, in particular to a strain of Pseudomonas fluorescens capable of degrading estrogen substances and its acquisition and application. technical background [0002] As a typical endocrine-disrupting substance in the environment, estrogen will accumulate and enrich in the organism in a direct or indirect manner; after binding to hormone receptors in the body, environmental estrogen will cause nervous system disorders, endocrine disorders, and immune competence in organisms. It has strong carcinogenicity and pathogenicity, directly threatens the survival and reproduction of human society and the biosphere, and has attracted widespread attention from academia and society. Estrone (E1), 17-β-estradiol (E2) and estriol (E3) are natural estrogens secreted by human and animal ovaries, which can be discharged into sewage treatment plants along with animal and human excrement , soil, farmland and other environments. T...

AI Technical Summary

Problems solved by technology

Conventional active sewage treatment systems cannot effectively remove estrogen in raw water. Estrogen is discharged into rivers along with sewage treatment plant effluent and accumulates in the environment

Studies have shown that ng / L levels of estrone and 17β-estradiol can be detected in rivers, causing secondary pollution to the environment

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