Method for biosynthesizing virginiamycin by streptomycete
A technology of virginiamycin and biosynthesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of virginiamycin having no domestic manufacturers, and improve feed utilization High efficiency, stable process and high fermentation yield
Examples
Embodiment 1
[0034] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:
[0035] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;
[0036] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;
[0037] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.
[0038] The concentration of each component of the slant nutrient medium is g / L: glucose 2.0, yeast extract 3.0, wort juice 8.0, MgSO 4 0.5, NaCl 0.5, K 2 PO 4 0.5, CaCO 3 2.0, agar 15.0, water balance, medium pH 6.7, culture ...
Embodiment 2
[0043] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:
[0044] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;
[0045] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;
[0046](3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.
[0047] The concentration of each component of the slant nutrient medium is g / L: glucose 3.0, yeast extract 4.5, wort juice 10.0, MgSO 4 0.8, NaCl 0.8, K 2 PO 4 0.8, CaCO 3 3.0, agar 23.0, water balance, medium pH 6.8, culture ...
Embodiment 3
[0052] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:
[0053] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;
[0054] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;
[0055] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.
[0056] The concentration of each component of the slant nutrient medium is g / L: glucose 4.0, yeast extract 6.0, wort juice 12.0, MgSO 4 1.0, NaCl1.0, K 2 PO 4 1.0, CaCO 3 4.0, agar 30.0, water balance, medium pH 7.0, culture ...
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Description
Claims
Application Information
- IPC
- C12P21/02; C12R1/465
- Inventors
- 韩风华; 李国栋