Method for biosynthesizing virginiamycin by streptomycete

A technology of virginiamycin and biosynthesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of virginiamycin having no domestic manufacturers, and improve feed utilization High efficiency, stable process and high fermentation yield

Active Publication Date: 2013-11-13
江西兴鼎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no domestic manufacturer of virginiamycin in the market, and it is mainly imported from Phipo Corporation of the United States.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0035] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0036] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0037] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0038] The concentration of each component of the slant nutrient medium is g / L: glucose 2.0, yeast extract 3.0, wort juice 8.0, MgSO 4 0.5, NaCl 0.5, K 2 PO 4 0.5, CaCO 3 2.0, agar 15.0, water balance, medium pH 6.7, culture ...

Embodiment 2

[0043] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0044] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0045] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0046](3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0047] The concentration of each component of the slant nutrient medium is g / L: glucose 3.0, yeast extract 4.5, wort juice 10.0, MgSO 4 0.8, NaCl 0.8, K 2 PO 4 0.8, CaCO 3 3.0, agar 23.0, water balance, medium pH 6.8, culture ...

Embodiment 3

[0052] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0053] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0054] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0055] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0056] The concentration of each component of the slant nutrient medium is g / L: glucose 4.0, yeast extract 6.0, wort juice 12.0, MgSO 4 1.0, NaCl1.0, K 2 PO 4 1.0, CaCO 3 4.0, agar 30.0, water balance, medium pH 7.0, culture ...

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Abstract

The invention discloses a method for biosynthesizing virginiamycin by streptomycete, which comprises the following steps of: preparing a slope with spores of virginiamycin; inoculating the slope spores into a culture medium to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation culture, adding a precursor substance at the early stage of fermentation so as to increase the yield of virginiamycin; and further processing the fermentation solution after fermentation to obtain virginiamycin crystals. The method disclosed by the invention is simple and easy to implement, is stable in the fermentation result and low in production cost. The fermentation result is improved compared with the traditional process, and the highest yield of virginiamycin can reach 3500 mu g / ml. Virginiamycin is polypeptide antibiotic containing lactonic rings, has the characteristics of no toxicity, no accumulation, easiness in degradation, and no drug resistance, and is a new animal feed additive.

Description

Technical field [0001] The present invention involves a method of synthesizing Virginycin in a chain mold biomedicin, which belongs to industrial biotechnology and fermented engineering fields. Background technique [0002] Virginia ( virginiamycin ) It is also known as hypertrophy, telithromycin, and vitamin, and is the Streptomycess virginiae ) The chain -positive antibiotic generated by fermentation is a type of beast antibiotics with novel structures, that is, polypeptide antibiotics containing lactone rings.Virginian is composed of two factors: M1 factor (about 70-80%) and S1 factor (about 20-30%).The M1 factor is a giant ring ester, and the molecular formula is C 28 H 35 N 3 O 7 , Especially for golden yellow bacteria, S1 factor is a ring -shaped polypeptide, and the molecular formula is C 43 H 49 N 7 O 10 , Especially effective for Tengya Bacteria.These two factors have synergistic effects. When M: S = 70: 30, its antibacterial activity is the highest, and it can produce a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 韩风华李国栋邹君彪邓健黄苓
Owner 江西兴鼎科技有限公司
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