Cecropin F protein antibacterial peptide and application thereof

A technology of cecropin and antibacterial peptide, applied in the field of biomedicine, can solve problems such as the unsatisfactory treatment effect of antibacterial drugs, and achieve the effect of preventing and resisting bacterial infection, promoting wound healing, and reducing the use of antibiotics

Active Publication Date: 2020-09-11
南京蓝色云生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the emergence of drug-resistant bacteria, the treatment effect of antibacterial drugs on wound infection is not satisfactory. Therefore, it is imminent to find new anti-infective drugs for wounds

Method used

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  • Cecropin F protein antibacterial peptide and application thereof
  • Cecropin F protein antibacterial peptide and application thereof
  • Cecropin F protein antibacterial peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Synthesis of embodiment 1 cecropin F protein antimicrobial peptide

[0035] S1. Weigh 1 times the molar equivalent of resin and put it into the reactor, add DCM (dichloromethane) to swell for half an hour, then remove the DCM, add 2 times the molar equivalent of the first amino acid in the sequence of SEQ ID NO.1, add 2 times Molar equivalent of DIEA, appropriate amount of DMF, DCM (appropriate amount means that the resin can be fully agitated), DIEA (diisopropylethylamine), DMF (dimethylformamide), DCM, nitrogen bubble reaction 60min. Then add about 5 times the molar equivalent of methanol, react for half an hour, remove the reaction solution, and wash with DMF and MEOH;

[0036] S2. Add the second amino acid in the sequence of SEQ ID NO.1 (also 2 times the molar equivalent) to the reactor, 2 times the molar equivalent of HBTU (1-hydroxy, benzo, trichlorazole tetramethyl hexafluorophosphate ) and DIEA, N2 bubbling reaction for half an hour, wash off the liquid, detec...

Embodiment 2

[0042] Example 2 Effects of CecF antimicrobial peptides on the propagation of Escherichia coli and Pseudomonas aeruginosa

[0043] 1. Preparation of CecF Antimicrobial Peptide Solution

[0044] Dissolve 0.5 mg of the CecF polypeptide synthesized in Example 1 in 500 ul sterile water to prepare 1.8 mg / ml and 0.18 mg / ml solutions, and place them in a -80 refrigerator for later use.

[0045] 2. Bacterial activation: Take 100ul of E. coli bacteria liquid and spread it on an LB (Luria-Bertani) plate without antibiotics, place it in a 37°C incubator, and incubate for 12 hours.

[0046]3. The application of CecF antimicrobial peptides to inhibit bacterial growth

[0047] (1) Take 100ul of the prepared CecF antimicrobial peptide solution, pick a single clone on the above-mentioned activated plate, put it in the antimicrobial peptide solution and mix it evenly, and then spread it on the LB plate without resistance. Incubate at 37°C for 12 hours, then remove for observation.

[0048] ...

Embodiment 3

[0054] Example 3 Effects of CecF Antimicrobial Peptides on Wound Healing in Mice

[0055] 1. Preparation of CecF Antimicrobial Peptide Solution

[0056] Dissolve 0.5 mg of CecF antimicrobial peptide synthesized in Example 1 in 2 ml of sterile water to make solutions of 400 ug / ml and 10 ug / ml, and place them in a -80 refrigerator for later use.

[0057] 2. The application of CecF antimicrobial peptide to promote wound healing

[0058] (1) Anesthesia: Kunming rats aged 6-8 weeks were selected, weighed, and injected intraperitoneally with chloral hydrate solution (10%) at 25 g / 100 ul.

[0059] (2) Shearing and depilation: After the mouse is anesthetized, fix it on the test bench, cut off the hair on the back and both sides of the mouse with scissors, apply the depilatory liquid evenly on the sheared area, wait for about 3-5 minutes, and use Gently wipe the skin with a cotton swab dampened with warm water to thoroughly remove the depilatory fluid and hair.

[0060] (3) Making w...

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Abstract

The invention discloses a cecropin F protein antibacterial peptide and an application thereof. An amino acid sequence of the cecropin F protein antibacterial peptide is as shown in SEQ ID NO.1, and anucleic acid sequence of a corresponding gene is as shown in SEQ ID NO.2. Experiments prove that the cecropin F protein antibacterial peptide disclosed by the invention has a strong inhibiting effecton escherichia coli and pseudomonas aeruginosa, can be used for preparing medicines for preventing and resisting bacterially infected wound repair and products for preventing and resisting bacterial infection in cosmetic related industries, and can also be used as a preservative for inhibiting bacteria in the food industry.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cecropin F protein antibacterial peptide and an application thereof. 【Background technique】 [0002] Previous studies have shown that antimicrobial peptides have inhibitory effects on bacteria, protozoa, and viruses. Antimicrobial peptides play an antibacterial role by acting on the bacterial cell membrane. Through the special mechanism of charge adsorption on the bacterial cell membrane, the target strain is not easy to mutate, so it is considered to have broad application prospects in the pharmaceutical industry. [0003] Antimicrobial peptides found in nature are mainly divided into defensin and cecropin. Cecropin was first discovered from the Drosophila genome, and has since been discovered in many species. Cecropin generally contains 37 to 39 amino acid residues and does not contain cysteine. Its N-terminal region is strongly basic and can form a nearly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N15/12A61K38/17A61P31/04A61P17/02A61K8/64A61Q19/00A61Q17/00A23L3/3526
CPCC07K14/43563A61P31/04A61P17/02A61K8/64A61Q19/00A61Q17/005A23L3/3526A23V2002/00A23V2200/10A23V2250/55Y02A50/30
Inventor 程功肖小平陈依玲
Owner 南京蓝色云生物科技有限公司
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