Application of poria cocos ethanol extract to biopesticides
A technology of ethanol extraction and poria cocos, which is applied in the direction of chemicals, biocides, and plant growth regulators for biological control, can solve the problems of no pesticide application, etc., and achieve the inhibition of plant pathogenic bacteria, low pollution, and easy degradation Effect
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Embodiment 1
[0019] The tested insects: cotton bollworm (Heliothis armigera), diamondback moth (Plutella xylostella) and bean aphid (Aphis craccivora), provided by Hubei Academy of Agricultural Sciences.
[0020] Test method: When Plutella xylostella and cotton bollworm were used as test insects, the eggs were sterilized with 10% formaldehyde solution for 15 minutes, and the improved feed formula of Liang Gemei of Beijing Academy of Agricultural Sciences was adopted, and the eggs were artificially raised indoors. Feeding conditions: temperature 25°C, relative humidity 75%-85%. Dilute the sample to a certain concentration and spread it on the surface of the artificial feed. A 96-well cell culture plate was used, and 300 μL of artificial feed and 20 μL of diluted drug solution were added to each well, and the experimental results were checked one week later. The concentration of the drug solution in the initial screening is 1.0 mg / mL, and the concentration in the secondary screening is adju...
Embodiment 2
[0024] Bacteria to be tested: Pythium ultimum, Botrytis cinerea, Septoria nodorum, Alternaria Solani, Fusarium culmorum, R. blight ( Rhizoctonia solani), provided by Hubei Academy of Agricultural Sciences.
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