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Medlar general flavones extracting and purifying technology and medlar general flavone detecting method as well as applications of medlar general flavone

A technology of total flavonoids and extraction process of wolfberry fruit is applied in the field of natural medicine and achieves the effect of low cost

Active Publication Date: 2015-02-18
陕西方舟制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most studies on wolfberry flavonoids focus on the crude extract level
In the published patent 201010542616.3, it is mentioned that the extraction and preparation method of flavonoids in wolfberry leaves adopts colloidal grinding technology, high-pressure homogenization technology and membrane separation combined application technology; but this method is an extraction method for extracting flavonoids in wolfberry leaves, and there is no An introduction to the extraction method of total flavonoids in wolfberry

Method used

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  • Medlar general flavones extracting and purifying technology and medlar general flavone detecting method as well as applications of medlar general flavone
  • Medlar general flavones extracting and purifying technology and medlar general flavone detecting method as well as applications of medlar general flavone
  • Medlar general flavones extracting and purifying technology and medlar general flavone detecting method as well as applications of medlar general flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Lycium barbarum was dried and pulverized at 50°C, weighed 50g into a round-bottomed flask, added 500ml of 80% ethanol, placed in a constant temperature water bath at 85°C for 2 extractions, 1 hour each time, combined the filtrates, concentrated and filtered When the volume reaches 200ml, add 3 times of 95% ethanol and let stand for 24h to filter with suction and concentrate the solution to 200ml. Extract the concentrated solution and petroleum ether at a ratio of 1:2 for 2-3 times, discard the petroleum ether layer, The crude extract of wolfberry flavonoids was obtained.

[0039] Purification process:

[0040] 1. Use macroporous resin D101 to purify the crude extract

[0041] Soak the macroporous resin in 95% ethanol for 1d in advance, then pack the column, wash it with distilled water until there is no alcohol smell, and load the obtained 200ml crude extract, the flow rate of the sample is 0.5-1.0ml / min, and the sample is finished Afterwards, it was eluted to colorle...

Embodiment 2

[0046] Lycium barbarum was dried and pulverized at 50°C, weighed 100g into a round-bottomed flask, added 1000ml of 80% ethanol, placed in a constant temperature water bath at 85°C for 2 extractions, 1 hour each time, combined the filtrates, concentrated and filtered When the volume reaches 200ml, add 3 times of 95% ethanol and let stand for 24h to filter with suction and concentrate the solution to 400ml. Extract the concentrated solution and petroleum ether at a ratio of 1:2 for 2-3 times, discard the petroleum ether layer, A crude extract of total flavonoids from Lycium barbarum was obtained.

[0047] Purification process:

[0048] 1. Use macroporous resin D101 to purify the crude extract

[0049] Soak the macroporous resin in 95% ethanol for 1 day in advance, then pack the column, wash it with distilled water until there is no alcohol smell, and load the obtained 400ml crude extract at a flow rate of 0.5-1.0ml / min. Afterwards, it was eluted to colorless with distilled wat...

Embodiment 3

[0054] Lycium barbarum was dried and pulverized at 65°C, weighed 200g into a round-bottomed flask, added 2000ml of 80% ethanol, placed in a constant temperature water bath at 85°C for 2 extractions, 1 hour each time, combined the filtrates, concentrated and filtered When the volume reaches 800ml, add 3 times of 95% ethanol and let stand for 24h to filter with suction and concentrate the solution to 800ml. Extract the concentrated solution and petroleum ether at a ratio of 1:2 for 2-3 times, discard the petroleum ether layer, A crude extract of total flavonoids from Lycium barbarum was obtained.

[0055] Purification process:

[0056] 1. Use macroporous resin D101 to purify the crude extract

[0057] Soak the macroporous resin in 95% ethanol for 1 day in advance, then pack the column, wash it with distilled water until there is no alcohol smell, and load the obtained 800ml crude extract at a flow rate of 0.5-1.0ml / min. Afterwards, it was eluted to colorless with distilled wat...

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Abstract

The invention belongs to a medlar general flavone extracting and purifying technology and a medlar general flavone detecting method as well as applications of medlar general flavone. At present, the method for preparing medlar general flavones comprises a reflux extracting method, an ultrasonic wave extracting method and a microwave extracting method. According to the extracting and preparing method of flavones in medlar leaves disclosed in 201010542616.3, a colloid breaking-in technique, a high-pressure homogenizing technique and a membrane separation and combination application technique are adopted, and the preparation is complex and long time is consumed. The invention aims to research the medlar general flavone extracting and purifying technology, the medlar general flavone detecting method as well as applications of the medlar general flavone. The technical scheme provided by the invention comprises the following steps of conducting an extracting technology: drying and crushing medlar, adding alcohol, extracting and combining filter liquor, concentrating, and extracting, thereby obtaining a medlar flavone rough extract; and conducting an extracting and purifying technology: purifying the rough extract by adopting porous resin D101, and further purifying the flavones by adopting polyamide. The medlar general flavone extracting and purifying technology, the medlar general flavone detecting method as well as applications of the medlar general flavone provided by the invention have the effects that general flavones are extracted as well as polysaccharide is obtained, effective ingredients of medlar are extracted fully, and foundation for large-scale industrial production is laid.

Description

technical field [0001] The invention belongs to the field of natural medicines, and relates to a wolfberry fruit extract with the function of scavenging free radicals, in particular to a method for extracting and purifying total flavonoids in wolfberry fruit, a detection method and an application thereof. Background technique [0002] Lycium Barbarum (Lycium Barbarum) is the dry and mature fruit of Lycium barbarum Ningxia, a plant of the Solanaceae family. It is a rare medicinal material and tonic, and it is also one of the traditional and important medicinal and edible homologous plants in China. Lycium barbarum is sweet in taste and flat in nature, and has the effect of nourishing the liver and kidney. Traditional Chinese medicine has long said that "lycium barbarum preserves health". "Compendium of Materia Medica" records: "Lycium barbarum, tonifies the kidney, nourishes the liver, nourishes the liver...improve eyesight and calm the nerves, and makes people live longer." ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/815A61P39/06G01N30/02A61K131/00
Inventor 王宇高凯杜小英汤海峰
Owner 陕西方舟制药有限公司
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