Method for cultivating and detecting mycoplasma pneumoniae

Abstract

The invention relates to a method for cultivating and detecting the mycoplasma pneumonia and belongs to the technical field of microbial culture. Devices of the method include a culture bottle I (100), a bottle cap (200) provided with a through hole (201) in the center and a culture bottle II (300); the culture bottle I (100) and the culture bottle II (300) are connected at two ends of the bottle cap (200); and a gasket (500), a filter membrane (600) and a filter membrane fixing unit (700) are further arranged in the bottle cap (200). According to the method, sputum samples are digested and revived through a digestion culture solution in the culture bottle I (100), and achieve initial multiplication, perform multiplication and enrichment through a selection bacteria enrichment solution in the culture bottle II (300), and the preliminary identification of the mycoplasma pneumonia is achieved; and finally the mycoplasma pneumonia is vaccinated to a solid medium of the culture bottle I (100) for re-multiplication, therefore, culture and identification of the mycoplasma pneumonia are completed. The devices of the method are designed aimed at the mycoplasma pneumonia, so that the mycoplasma pneumonia is rapid and convenient to culture, the result accuracy is high, and needs of clinicalsputum sample culture and detection can be met.

Description

Technical field [0001] The present invention involves the cultivation and detection method of a chlamydia pneumoniac, which belongs to the field of microbial training technology. Background technique [0002] Pneumonia (M. Pneumonia) is one of the important pathogenic microorganisms of human acute respiratory tract infections. It is mainly transmitted through the respiratory tract and droplets, which can cause school -age children and adolescents down respiratory infections, such as atypical pneumonia, bronchitis, bronchitis, etc.;It can also cause external complications of other systems, such as meningitis, encephalitis, myocarditis, pericarditis, nephritis.Pneumoniacura is a classic microorganism that lacks cell walls and is different from cells and viruses.There are many types and a wide range of distribution. The size is generally between 0.2-0.3um and can be passed through a general bactericide filter. [0003] The clinical symptoms of chlamydia infection of pneumoniacus are...

AI Technical Summary

Problems solved by technology

In general, the above method not only takes a long time to detect and is cumbersome to operate, but also requires some sophisticated instruments and equipment, and requires professional and technical personnel to conduct experiments, which is inconvenient to use

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