Molecular motor biosensor kit for detecting vibrio parahaemolyticus

Abstract

The invention provides a molecular motor biosensor kit for detecting vibrio parahaemolyticus, which belongs to the field of rapid detection of food microorganisms. The invention mainly aims to overcome the problem of a long period of detection time (about 4 days) in a traditional detection method. The core technology of the invention is to employ an F0F1-ATPase molecular motor biosensor on a chromatophore; since F0F1-ATP synthase can rotate at a fast speed, coupling between a catalysis site and proton transfer is established through rotation of the F0F1-ATP synthase. According to the invention, a toxR probe is connected with epsilon subunit of the ATP synthase at first; a to-be-detected sample and a negative control are respectively combined with the biosensor; after the to-be-detected sample and the negative control have been used for catalytic synthesis of ATP for 30 min, yield of ATP is compared, wherein the amount of synthesized ATP can be measured by measuring the content of H<+> in an environment, and the content of H<+> is obtained through fluorescence intensity represented by H-DHPE. The kit can realize rapid, sensitive and high flux detection of vibrio parahaemolyticus in the to-be-detected sample.

Description

technical field [0001] The invention relates to a rapid detection technology for food microorganisms. Background technique [0002] The traditional detection method of Vibrio parahaemolyticus requires steps of non-selective enrichment, selective enrichment, separation, screening and identification for each inspection item. It usually takes 4 days to issue a test report, which seriously affects the quality and quality of the goods. shelf life. Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection. This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safet...

AI Technical Summary

Problems solved by technology

[0002] The traditional detection method of Vibrio parahaemolyticus requires steps of non-selective enrichment, selective enrichment, separation, screening and identification for each inspection item. It usually takes 4 days to issue a test report, which seriously affects the quality and quality of the goods. shelf life

Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection

This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government departments.

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