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Method for detecting trace amount of oxytetracycline by utilizing CdTe quantum dot fluorescent probe

A fluorescent probe, oxytetracycline technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., to achieve the effect of improving sensitivity and selectivity, convenient and fast detection

Inactive Publication Date: 2013-05-01
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of CdTe quantum dot fluorescent probes in the detection of oxytetracycline has not been reported yet.

Method used

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  • Method for detecting trace amount of oxytetracycline by utilizing CdTe quantum dot fluorescent probe
  • Method for detecting trace amount of oxytetracycline by utilizing CdTe quantum dot fluorescent probe
  • Method for detecting trace amount of oxytetracycline by utilizing CdTe quantum dot fluorescent probe

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Experimental program
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Embodiment Construction

[0019] 1. Preparation of CdTe quantum dots:

[0020] (1) Weigh 0.0480g tellurium powder and 0.12g NaBH 4 Place in a clean three-necked flask, mix well, add 2.0 mL of secondary deionized water, react in a 65°C water bath and magnetic stirring for 20 minutes, and obtain a purple transparent NaHTe aqueous solution.

[0021] (2) Under nitrogen protection, in 200 mL of CdCl with a concentration of 0.0025 mol / L 2 Add 0.10 mL of analytically pure thioglycolic acid to the aqueous solution, adjust the pH to 10, quickly add 2.0 mL of the NaHTe aqueous solution obtained in the first step of step (1) under strong stirring, and reflux at 95°C for 2 hours to obtain a clear and transparent aqueous solution. Sexual CdTe quantum dots.

[0022] (3) Put the CdTe quantum dots prepared in the second step of step (1) into dialysis bags, dialyze overnight with Tris-HCl buffer solution with pH=8.6, dry the dialyzed solution in vacuum, and then use pH=8.6 Tris-HCl buffer solution is dissolved to pr...

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Abstract

The invention discloses a method for detecting a trace amount of oxytetracycline by utilizing a CdTe quantum dot fluorescent probe. According to the invention, CdTe quantum dots are taken as the fluorescent probe, and oxytetracycline and CdTe quantum dots are utilized to be combined to form a new composite system through electrostatic interaction to cause the quenching of fluorescent light of CdTe quantum dots, so that a method for detecting the trace amount of oxytetracycline is established. A favorable linear relation is formed by the quenching amount of fluorescent light of CdTe quantum dots and the concentration of gibberellin within the concentration range of 8*10<-9>-2*10<-7> mol / L, and the detection limit of the method is 2.72*10<-10> mol / L. According to the invention, defects of low sensitivity, long reaction time, poor stability and narrow application range during detecting in the prior art are overcome, the sensitivity and selectivity are better improved, and the detection to the low-concentration oxytetracycline is more convenient and faster.

Description

technical field [0001] The invention relates to a method for quickly measuring trace oxytetracycline by using CdTe quantum dot probe technology combined with fluorescence quenching method. Background technique [0002] Oxytetracycline (Oxytetracycline) belongs to tetracycline broad-spectrum antibiotics, mainly used for rickettsiasis, brucellosis, mycoplasma pneumonia, chlamydia infection, and can also be used for mild infection caused by sensitive Gram-positive cocci and negative bacilli . It is widely used in animal medicine and feed additive in agriculture and animal husbandry. However, studies have shown that long-term use of large doses of oxytetracycline causes certain residues in animal foods, and enters the human body through the food chain, endangering human health. At present, many countries have made strict regulations on the residue limit of oxytetracycline in food of animal origin. Therefore, it is of great significance to study a highly sensitive and highly s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 陶慧林黎舒怀徐铭泽曾秋莲
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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