Application of Roseburia to treatment and prevention of obesity related disease
A Rothella, the use of technology, applied in the field of microbiology, can solve the problems of immune resistance, calculation errors, small side effects, treatment and prevention of obesity and its related diseases
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Embodiment 1
[0068] Experimental Materials
[0069] Mice: C57BL / 6J mice (purchased from the Experimental Animal Center of Southern Medical University) were purchased, which were 6-week-old normally bred mice, 15 in total. Mice were raised in the same environment and fed the same food.
[0070] Strain: Roseburia inulinivorans DSM 16841 strain, purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH).
[0071] High-fat food (HF): containing 58% lard, 25.6% carbohydrate, and 16.4% protein, purchased from Beijing Huafukang Biotechnology Co., Ltd.
[0072] Conventional mouse food: purchased from the Animal Experiment Center of Southern Medical University.
Embodiment 2
[0074] Grouping and induction of mice
[0075] In this embodiment, 3 experimental groups were set up, with 5 animals in each group.
[0076] 6-week-old C57BL / 6J mice fed normally (no need to be sterile) were selected for the experiment. The DSM 16841 bacteria to be tested were introduced into the intestinal tract of the mice by feeding method, and the insulin resistance ability of the mice was identified by the glucose tolerance (OGGT) test.
[0077] For specific experimental grouping information and processing steps, see figure 1 .
[0078] The 15 repurchased mice were kept in the same environment until they were 6 weeks old (the food did not need to be sterilized), the amount of food fed was recorded, and the body weight and physical condition of the mice were regularly monitored.
[0079] The 15 mice were equally divided into 3 experimental groups (5 replicates in each experimental group), placed in ultra-clean workbenches, and formally entered the experimental period. Th...
Embodiment 4
[0084] Experimental results:
[0085] 1. The body weight gain results of feeding mice for 10 weeks are shown in Table 1 and figure 2 .
[0086] Table 1
[0087]
[0088] 2. The comparison results of the P value of the difference in body weight growth of mice fed for 10 weeks are shown in Table 2 and image 3 .
[0089] Table 2
[0090]
[0091] From Table 1, Table 2 and figure 2 , image 3 It can be seen that feeding the test bacteria can significantly reduce the body weight of obese mice after feeding for 10 weeks.
[0092] 3. See Table 3, Table 4 and Figure 4 , Figure 5 .
[0093] table 3
[0094] group
[0095] 1.
[0096] Table 3 and Figure 4 It was shown that feeding the test bacteria can significantly reduce the blood sugar of obese mice.
[0097] Table 4
[0098] P value
[0099] Table 4 and Figure 5 It was shown that feeding the test bacteria can significantly improve the blood sugar tolerance of obese mice.
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