Breeding method of laying hen with low egg yolk cholesterol content

A cholesterol and egg yolk technology, which is applied in the field of poultry genetics and breeding, can solve the problems of additives or drug residues, egg yolk cholesterol content for a long time, etc., and achieve the effect of reducing feeding amount, shortening generation interval, and improving selection accuracy

Inactive Publication Date: 2013-05-29
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a breeding method for laying hens with low egg yolk cholesterol content, so as to solve the problems of reducing egg yolk cholesterol content for a long time and residual additives or drugs

Method used

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  • Breeding method of laying hen with low egg yolk cholesterol content
  • Breeding method of laying hen with low egg yolk cholesterol content
  • Breeding method of laying hen with low egg yolk cholesterol content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Extraction of sample chicken DNA

[0026] In the breeding base group, 500 sample chickens were selected to be reared in single cages, and the DNA of the sample chickens was extracted. The steps are as follows:

[0027] 1) DNA extraction

[0028] a) Add 500 μL of TE buffer, 12 μL of SDS lysate (25%) and 10 μL of proteinase K (10 mg / L) to 80 μL of whole blood, shake and mix for 30 minutes, and digest in a water bath at 57°C overnight;

[0029] b) Add 602 μL of Tris saturated phenol, shake well for 10 minutes, and centrifuge at 10,000 r / min for 10 minutes;

[0030] c) Take the supernatant, add 500 μL of Tris saturated phenol, vortex for 10 minutes, and centrifuge at 10,000 r / min for 10 minutes;

[0031] d) Take the supernatant, add an equal volume of phenol / chloroform / isoamyl alcohol (25:24:1), shake slowly for 10 minutes, and centrifuge at 1,2000r / min for 10 minutes;

[0032] e) Take the supernatant, add an equal volume of chloroform / isoamyl alcohol (24:1), shake sl...

Embodiment 2

[0074] The sample chickens of selected genotypes in Example 1 were selected for pure line selection by the closed group breeding method, and 50 families were established, with a male-to-female ratio of 1:10. Individual cages were raised to 40 weeks of age, and the production rate of each hen was recorded. Egg number, average egg weight and egg yolk cholesterol content at the age of 40 weeks, calculated the mean value of egg number, egg weight and egg yolk cholesterol content of all hens in 50 families and hens in each family, as shown in Table 5. Taking egg yolk cholesterol content as the main trait, taking egg weight and egg production into consideration, selecting the top 13 families whose egg yolk cholesterol content was lower than the group average, and egg weight and egg production higher than the group average, to breed the next generation, see Table 6 .

[0075] Table 550 families of all hens and the average value of the three traits of egg number, egg weight and egg yo...

Embodiment 3

[0082] Extract the DNA of the 2-week-old offspring of the family chickens selected in Example 2, and carry out PCR-RFLP analysis with a pair of primers in Example 1. According to the HMGCR gene molecular marker g.12217G>T of the candidate individual, the GG type is selected for early Molecular marker-assisted selection.

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Abstract

The invention discloses a breeding method of a laying hen with low egg yolk cholesterol content. The breeding method comprises the following steps of (1) taking a DNA (deoxyribonucleic acid) sequence including chicken HMGCR (Hydroxymethyl glutaric acyl coenzyme) gene as a template, designing a pair of primers, and carrying out sample chicken individual gene parting by a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method; (2) utilizing a genetic effect analysis model to determine selected and remained genetype; (3) building a family from individual bodies of cocks and hens according with the selected and remained genetype; (4) extracting the DNA in later generation of the selected and remained family hen, carrying out PCR-RFLP analysis on the pair of primers in step (1), selecting and remaining the hens with selected and remained genetype in step (2); and (5) repeating steps (3) and (4) to continuously breed for at least three generations, so as to obtain the laying hen with low egg yolk cholesterol content. The HMGCR gene molecular marker is adopted to assist and select the breeding method; the generation interval is shortened; the breeding accuracy is improved; the breeding flock feeding capacity is reduced; and the feeding cost is also reduced.

Description

technical field [0001] The invention relates to a breeding method for laying hens with low egg yolk cholesterol content, and belongs to the technical field of poultry genetics and breeding. Background technique [0002] Cholesterol is an important part of various membrane phase structures of animal cells and nerve myelin sheath. It is the precursor of bile acids, steroid hormones and vitamin D3, and has important physiological functions. However, if the body takes in a large amount of cholesterol, its content in the blood will increase significantly, and this is one of the important factors that cause cardiovascular diseases such as coronary arteriosclerosis and cerebral thrombosis. The American Heart Association recommended in the early 1970s that cholesterol intake should be less than 300 mg per day. Dietary cholesterol comes from animal foods, and eggs are one of the higher cholesterol content in animal foods. Studies have shown that cholesterol in eggs is an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 田亚东康相涛魏杨李国喜孙桂荣韩瑞丽
Owner HENAN AGRICULTURAL UNIVERSITY
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