Breeding method of laying hen with low egg yolk cholesterol content
A cholesterol and egg yolk technology, which is applied in the field of poultry genetics and breeding, can solve the problems of additives or drug residues, egg yolk cholesterol content for a long time, etc., and achieve the effect of reducing feeding amount, shortening generation interval, and improving selection accuracy
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Embodiment 1
[0025] 1. Extraction of sample chicken DNA
[0026] In the breeding base group, 500 sample chickens were selected to be reared in single cages, and the DNA of the sample chickens was extracted. The steps are as follows:
[0027] 1) DNA extraction
[0028] a) Add 500 μL of TE buffer, 12 μL of SDS lysate (25%) and 10 μL of proteinase K (10 mg / L) to 80 μL of whole blood, shake and mix for 30 minutes, and digest in a water bath at 57°C overnight;
[0029] b) Add 602 μL of Tris saturated phenol, shake well for 10 minutes, and centrifuge at 10,000 r / min for 10 minutes;
[0030] c) Take the supernatant, add 500 μL of Tris saturated phenol, vortex for 10 minutes, and centrifuge at 10,000 r / min for 10 minutes;
[0031] d) Take the supernatant, add an equal volume of phenol / chloroform / isoamyl alcohol (25:24:1), shake slowly for 10 minutes, and centrifuge at 1,2000r / min for 10 minutes;
[0032] e) Take the supernatant, add an equal volume of chloroform / isoamyl alcohol (24:1), shake sl...
Embodiment 2
[0074] The sample chickens of selected genotypes in Example 1 were selected for pure line selection by the closed group breeding method, and 50 families were established, with a male-to-female ratio of 1:10. Individual cages were raised to 40 weeks of age, and the production rate of each hen was recorded. Egg number, average egg weight and egg yolk cholesterol content at the age of 40 weeks, calculated the mean value of egg number, egg weight and egg yolk cholesterol content of all hens in 50 families and hens in each family, as shown in Table 5. Taking egg yolk cholesterol content as the main trait, taking egg weight and egg production into consideration, selecting the top 13 families whose egg yolk cholesterol content was lower than the group average, and egg weight and egg production higher than the group average, to breed the next generation, see Table 6 .
[0075] Table 550 families of all hens and the average value of the three traits of egg number, egg weight and egg yo...
Embodiment 3
[0082] Extract the DNA of the 2-week-old offspring of the family chickens selected in Example 2, and carry out PCR-RFLP analysis with a pair of primers in Example 1. According to the HMGCR gene molecular marker g.12217G>T of the candidate individual, the GG type is selected for early Molecular marker-assisted selection.
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