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Artificial synthetic adjuvant for avian influenza intranasal immunization

An artificial synthesis and immune adjuvant technology, which is applied in antiviral agents, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of muscle quality reduction, affecting animal growth, etc., to improve the level of cellular immunity and reduce costs. Low, reduced stress and effects on meat quality

Inactive Publication Date: 2013-06-05
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the stress response caused by intramuscular injection affects the growth of animals. In addition, the oil emulsion injected into the muscle can also cause a decrease in muscle quality

Method used

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  • Artificial synthetic adjuvant for avian influenza intranasal immunization
  • Artificial synthetic adjuvant for avian influenza intranasal immunization
  • Artificial synthetic adjuvant for avian influenza intranasal immunization

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Embodiment Construction

[0032] Part 1: Preliminary Tests

[0033] 1. Design of CpG-ODN sequence

[0034]According to the relationship between the immune activity of CpG-ODN and its sequence structure characteristics shown in the current research, several CpG-ODN sequences were designed using the sequence CpG-ODN2006 (positive sequence) as a template, involving the sequence in the CpG-ODN sequence. Length, backbone modification, CpG base sequence and the number of positions of the motif, base characteristics of the 5' end and 3' end of the sequence, presence or absence of palindrome structure in the sequence, etc. CpG-ODN sequence see figure 1 .

[0035] 2. The stimulating effect of CpG-ODN on chicken spleen lymphocytes

[0036] Firstly, chicken spleen lymphocytes were isolated in vitro, added different sequences of CpG-ODN to stimulate them and cultured for 3 days, and the stimulating effect of each CpG-ODN on chicken spleen lymphocytes was detected by CCK-8 method. It was found that the stimulat...

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Abstract

The invention relates to an artificial synthetic adjuvant for avian influenza intranasal immunization, which belong to the technical field of biology and is specially used for applying avian influenza inactivated virus intranasal immunization. The function of CpG oligodeoxynucleotide (ODN) is closely related to the sequence and structural features. A plurality of CpG-ODN sequences are designed according to the relationship and the CpG-ODN sequence with an optimum immunologic adjuvant effect is screened through internal and external tests. An adopted formula is as follows: the sequence of 10-100mug of CpG-ODN F3 of each chick is 5'-TCGTCGTTGTCGTTTTGTCGTTGGGGGG-3'. The local mucous membrane immunization and whole humoral immunization levels can be effectively improved under the cooperation with avian influenza inactivated virus through intranasal immunity, a muscle injection immunization process is avoided, the chicken stress reaction is reduced, and guarantee is provided for improving chicken quality. Moreover, the CpG-ODN cost is lowered, and the possibility of widely applying the artificial synthetic adjuvant to poultry vaccines is increased.

Description

1. Technical field [0001] The invention relates to an artificially synthesized nasal cavity immune adjuvant (nasal cavity mucosal immunity) for avian influenza inactivated virus, which belongs to the field of biotechnology and is specially used for improving the effect of nasal cavity mucosal immunity of avian influenza inactivated virus. 2. Background technology [0002] Features of nasal immunity [0003] Mucosal immunity is the immunity on the mucosal surface of the cavity (respiratory tract, digestive tract, reproductive tract, etc.) that the body communicates with the outside world. It is the first barrier for animals to defend against external pathogenic microorganisms. Common mucosal immunization routes include oral immunization, nasal cavity immunization, and vaginal immunization. Among them, nasal immunization is an attractive immune route. It has been widely valued for its following advantages: the physiological environment of the nasal cavity is mild, and the th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61P31/16
Inventor 杨倩付佳庾庆华
Owner NANJING AGRICULTURAL UNIVERSITY
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