Culture medium for efficient induction of garlic calli

A technology of callus and medium, which is applied in the field of high-efficiency induction medium of garlic callus, and can solve the problem of low induction rate of garlic bulb tip tissue

Inactive Publication Date: 2013-06-12
王茜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a medium for efficient induction of garlic callus, which overcomes the problem of low induction rate of ga

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. The common white-skinned garlic purchased from the market to break the dormancy period was used as the test plant material. Forchlorfenuron, sodium nitrophenolate, and hydrolyzed casein are all products of Beijing Suo Laibao Technology Co., Ltd., and other reagents used in the experiment are of domestic analytical grade;

[0020] 2. Peel off the white skin garlic. Peel off the garlic cloves and place them in a 12cm-diameter petri dish. Put 5 layers of gauze under it, soak it in water, place the peeled garlic cloves on the gauze, and root them in hydroponics at 25°C. Change the water once;

[0021] 3. Remove the healthy fresh root tip of 3 mm and wash it with plenty of water, then soak it in 75% alcohol for 10 seconds, then put it in 0.1% mercury chloride for 7 minutes, and then wash it with plenty of sterile water for 8 times to remove the residual disinfectant;

[0022] 4. Connect the above-mentioned explants after disinfection to the medium containing forchlorfenu...

Embodiment 2

[0024] 1. The common white-skinned garlic purchased from the market to break the dormancy period was used as the test plant material. Forchlorfenuron, sodium nitrophenolate, and hydrolyzed casein are all products of Beijing Suo Laibao Technology Co., Ltd., and other reagents used in the experiment are of domestic analytical grade;

[0025] 2. Peel off the white skin garlic. Peel off the garlic cloves and place them in a 12cm-diameter petri dish. Put 5 layers of gauze under it, soak it in water, place the peeled garlic cloves on the gauze, and root them in hydroponics at 25°C. Change the water once;

[0026] 3. Rinse the healthy fresh root tip of 4 mm with plenty of water, then soak it in 75% alcohol for 12 seconds, then put it in 0.1% mercury chloride for 7 minutes, and then rinse it with plenty of sterile water for 9 times to remove the residual disinfectant;

[0027] 4. Connect the above-mentioned explants after disinfection to the medium containing forchlorfenuron, sodium ...

Embodiment 3

[0029] 1. The common white-skinned garlic purchased from the market to break the dormancy period was used as the test plant material. Forchlorfenuron, sodium nitrophenolate, and hydrolyzed casein are all products of Beijing Suo Laibao Technology Co., Ltd., and other reagents used in the experiment are of domestic analytical grade;

[0030] 2. Peel off the white skin garlic. Peel off the garlic cloves and place them in a 12cm-diameter petri dish. Put 5 layers of gauze under it, soak it in water, place the peeled garlic cloves on the gauze, and root them in hydroponics at 25°C. Change the water once;

[0031] 3. Remove 5 mm of strong fresh root tips and rinse with plenty of water, then soak in 75% alcohol for 15 seconds, then put in 0.1% mercury chloride for 8 minutes, and then rinse with plenty of sterile water for 10 times to remove residual disinfectant;

[0032] 4. Connect the above-mentioned explants after disinfection to the medium containing forchlorfenuron, sodium nitro...

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PUM

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Abstract

The invention discloses a culture medium for efficient induction of garlic calli, relating to a technology for garlic tissue culture, and belongs to the technical field of agriculture. The culture medium is characterized in that the dosage of every component on the basis of the added amount of MS in 1L of a basic culture medium of is as follows: 1L of MS, 1.0-2.0mg of forchlorfenuron, 0.05-0.5mg of compound sodium nitrophenolate, 200mg of casein hydrolysate, 20g of sucrose and 6g of agar. The culture medium has the beneficial effects that the culture medium has the functions of promoting cell division and expansion, the organofaction and synthesis of protein, improving the efficiency of photosynthesis, enhancing stress resistance, delaying senescence, promoting flower bud differentiation on melon and fruit plants, protecting flowers and fruits, improving the fruit setting rate, promoting fruit expansion, and the like; and the use of a compound plant growth regulator ensures that the culture medium can quickly permeate into plant bodies after contacting with plants.

Description

technical field [0001] The invention relates to garlic tissue culture technology, belongs to the field of agricultural technology, in particular to a medium for efficiently inducing garlic callus. Background technique [0002] Garlic, perennial herb, Liliaceae Allium. The underground bulbs are divided into petals, and are divided into purple-skinned species and white-skinned species according to different skin colors. Spicy, with a pungent smell, it can be eaten or seasoned, and can also be used as medicine. Garlic is rich in nutrients: every 100 grams contains 69.8 grams of water, 4.4 grams of protein, 0.2 grams of fat, 23.6 grams of carbohydrates, 5 mg of calcium, 44 mg of phosphorus, 0.4 mg of iron, and 3 mg of vitamin C. In addition, it also contains thiamine, riboflavin, niacin, allicin, citral, and trace elements such as selenium and germanium. Contains about 0.2% volatile oil. The main ingredient in the oil is allicin, which has a bactericidal effect. It is produc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王茜
Owner 王茜
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