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A technology of mountain green tea and seedlings, applied in the field of plant tissue culture, can solve the problems of long breeding period, low survival rate, low germination rate, etc., and achieve the effect of maintaining excellent traits, high transplanting survival rate, and stable genetic characteristics
Active Publication Date: 2014-04-23
广西万寿堂药业有限公司
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Problems solved by technology
At present, wild mountain green tea is mainly propagated by seeds, and its seed embryo tissue is poorly developed. It is also affected by factors such as season and growth environment. , the survival rate is low, and the reproduction is limited by natural conditions and many other problems, which cannot meet the needs of large-scale artificial cultivation.
[0004] After searching, so far, there are no domestic reports on the breeding and cultivation of camellia green tea, and there are no relevant research records abroad.
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Effect test
Embodiment 1
[0025] (1) Selection and sterilization of explants
[0026] Take the ripe berries of camellia green tea, soak them in 0.05% detergent solution for 10 minutes, then rinse them with running water for 15 minutes, and then wash them with 0.1% HgCl 2 Disinfect the solution for 10 minutes, then use tweezers to peel off the pericarp and take the seeds to inoculate in the seed germination medium for germination culture. The temperature of the culture room is 24-26°C, and the seeds are sterilized by this method without pollution;
[0027] Seed germination medium is MS+1.5mg·L -1 6-BA+0.5mg·L -1 NAA+sucrose 25g·L -1 +Agar 5g·L -1 , pH6.0.
[0028] (2) Induction of the first generation
[0029] The seeds began to germinate on 20 days (the germination rate was 83.2%). After the cotyledons unfolded, the root system of the aseptic seedlings was cut off and transferred to the cluster bud induction medium for cultivation. The cultivation was carried out for 10 days (temperature 24-26°C, ...
Embodiment 2
[0040] Others are basically the same as in Example 1, only with 0.1%HgCl in step (1) 2 After the solution was disinfected for 5 minutes, the contamination rate was 16.7%, the germination rate was 83.4%, the induction rate was 98.7%, the multiplication factor was 12.7, the rooting rate was 92%, and the transplanting survival rate was 85.8%.
Embodiment 3
[0042] Others are basically the same as in Example 1, only the seed germination medium in step (1) is MS+0.5mg L -1 6-BA+1.0mg·L -1 NAA+sucrose 30g·L -1 +Agar 5g·L -1 , pH 6.0; the temperature of the culture room was 26-28°C, and the results began to germinate after 20 days, the germination rate was 84.6%, the induction rate was 98.5%, the multiplication factor was 12.5, the rooting rate was 91%, and the transplanting survival rate was 86.1%.
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Abstract
The invention discloses a rapid breeding method of green tea sprout. The rapid breeding method of green tea sprout comprises the steps of explant sterilization, primary-culture induction, subculture multiplication and rooting transplanting. Green tea can be multiplied rapidly, a great amount of high-quality seedlings are produced within short time, the sprout can be directly used in the field production, and the situation of insufficient source of feed and short supply of the green tea is alleviated. The rapid breeding method is low in production cost, simple and convenient to operate, high in breeding speed and short in period, a great amount of high-quality green tea test-tube plantlets can be obtained rapidly and continuously in the whole year, and large-scale industrial production and application is achieved. By the breeding method of green tea sprout, the survival rate of transplanting is high, the hereditary character of the sprout is stable, heteromorphosis is avoided, excellent character of the sprout can be kept, and the use safety of the sprout is improved. The rapid breeding method has positive influence on the regeneration and continuous use of medicinal plant resources, and can be widely applied to commercialized production.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid breeding method for camellia seedlings. Background technique [0002] Mountain green tea is processed from the dried leaves of Ilex hainanensis Merr. Mountain green tea has clear curative effect and a long history of use, which has been included in "Guangxi Standards of Chinese Medicinal Materials" (1990 edition). Mountain green tea has the effects of clearing heat and detoxifying, reducing swelling and pain, promoting blood circulation and dredging channels. It is mainly used for lowering blood pressure, blood fat, cholesterol, coronary heart disease, hemiplegia caused by cerebrovascular accident, wind-heat cold, lung-heat cough, and throat edema. , tonsillitis, dysentery and other diseases. Pharmacological studies have also shown that triterpenoids in camellia green tea have anti-tumor, anti-atherosclerosis, anti-hepatic virus, anti-inflammato...
Claims
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