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Targeting liver cell carrier and preparation method thereof

A liver cell and carrier technology, which is applied in pharmaceutical formulations, liposome delivery, drug combination and other directions, can solve the problems of complex preparation, high toxicity and high immunogenicity, and achieves improved enrichment, improved targeting, Enhanced therapeutic effect

Active Publication Date: 2013-06-12
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Viral vectors have high transfection efficiency, but there are disadvantages such as high immunogenicity, high toxicity, small target gene capacity, poor targeting specificity, complicated preparation and high cost.

Method used

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  • Targeting liver cell carrier and preparation method thereof
  • Targeting liver cell carrier and preparation method thereof
  • Targeting liver cell carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The carrier targeted for introduction into liver cells is a complex composed of liposomes and apolipoprotein ApoE. The complex is a kind of non-viral carrier with cell membrane penetration. The liposome is an artificially prepared phospholipid vesicle with a double-layer envelope, because ApoE can specifically recognize receptors on the liver cell membrane, thereby mediating the combination of liposome and liver cell membrane, which is beneficial to the target of the carrier. tropism.

[0025] The specific preparation method of carrier comprises the following steps:

[0026] 1. Preparation of cationic liposomes

[0027] Weigh 100 mg each of didodecyldimethylammonium bromide (DDAB, AVANTI POLAR LIPIDS, INC.) and dioleoylphosphatidylethanolamine (DOPE, AVANTI POLAR LIPIDS, INC.), dissolve in 1.2ml absolute ethanol Inject it into 12ml of water for injection at 60°C.

[0028] Squeeze with polycarbonate membranes with specifications of 150-200nm, 80-120nm and 40-60nm res...

Embodiment 2

[0038] Study on the Efficiency of Transfecting Liver Cancer Cells with Carriers Targeting Liver Cells

[0039] 1. Cell culture

[0040] Test cells: human liver cancer cells SMMC7721, normal human liver cells L-02, cardiomyocytes H9c2.

[0041] SMMC7721 was cultured in RPMI1640 containing 10% fetal bovine serum (GIBCO) in vitro, and other cells were cultured in high-glucose DMEM (GIBCO) containing 10% fetal bovine serum.

[0042] 2. Transfection efficiency of carrier complexes in liver cancer cell lines

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Abstract

The invention provides a carrier for introducing genes into liver cells in a targeting mode and a preparation method thereof. The carrier is a compound consisting of lipidosome and apolipoprotein, wherein the lipidosome is prepared from bidodecyl dimethylammonium bromide and dioleoyl phosphatidyl ethanolamine and passes through double-layer coated phospholipids follicle modified by distearoyl phosphatidyl ethanolamine-polyethylene glycol-maleimide; and the apolipoprotein is ApoE. According to the constructed targeting gene carrier, the targeting property of the carrier is improved through the affinity concentration of ligand-receptor, the transfection efficiency and the concentration degree in the liver cells are improved, and the carrier has obvious liver cell targeting property and can serve as artificial membranes and excipients of various treatment agents, so that the curative effect of drugs is enhanced.

Description

technical field [0001] The invention relates to a liposome carrier, in particular to a liposome carrier capable of specifically targeting liver cells and a preparation method thereof. Background technique [0002] Liver cancer is one of the common tumors, and its incidence is increasing worldwide, seriously endangering human life and health. The current treatment of liver cancer is nothing more than chemotherapy or surgical treatment, but the effect is not very satisfactory. With the increasing development of gene technology, it is possible to treat liver cancer from the aspect of gene, and the technology is becoming more and more mature. How to realize the targeting of gene therapy is an urgent problem to be solved in current research. [0003] The vectors used in gene therapy mainly include viral vectors and non-viral vectors. Viral vectors have high transfection efficiency, but they have disadvantages such as high immunogenicity, high toxicity, small target gene capaci...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/42A61K9/127A61P35/00
Inventor 于保锋牛栓成郭睿解军张小曼李春锋高然朋刘丹郝宇卉牛璐婷梁样红杨丽娟胡晓年向前张悦红陈显久
Owner SHANXI MEDICAL UNIV
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