Preparation method of compound polysaccharide consisting of schizophyllum commune exopolysaccharide and oat polysaccharide

A technology of extracellular polysaccharides and oat polysaccharides, which is applied in the field of compound polysaccharide preparation, can solve problems affecting product quality and fermentation success or failure, affecting the quality and appearance of final products, and slow growth of fungal mycelium, etc., so as to save equipment costs and production Cycle shortening, good immune boosting effect

Inactive Publication Date: 2013-06-19
徐宝军
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Problems solved by technology

Under normal circumstances, the fermentation product will also have unsatisfactory color and smell, which will affect the quality and appearance of the final product
[0005] Secondly, because fungal mycelium grows slowly in submerged culture, the chance of encountering contamination during the process will also increase, resulting in losses. Therefore, the quality of the liquid strain and the amount of inoculum will affect the quality of the final product and the success of fermentation

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  • Preparation method of compound polysaccharide consisting of schizophyllum commune exopolysaccharide and oat polysaccharide

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Embodiment Construction

[0027] Below in conjunction with embodiment the present invention is described in further detail.

[0028] A preparation method of a complex polysaccharide composed of schizophyllum exopolysaccharide and oat polysaccharide, which comprises the following steps:

[0029] Step 1: Activation of strains: Inoculate Schizophyllum sp. on PDA plate medium for rejuvenation and activation culture to obtain plate strains;

[0030] Step 2: Cultivation of the primary bacterial strain: in the fermenter, inoculate the cultivated plate bacterial strain into the seed medium, and cultivate it on a shaker to obtain the primary bacterial strain;

[0031] Step 3: Cultivation of the secondary strains: In the fermenter, the primary strains are inoculated in the seed medium, cultivated on a shaker to obtain the secondary strains;

[0032] Step 4: Liquid fermentation of strains: In the fermenter, inoculate the secondary strains on the basic medium, stir and ferment at a constant speed, and detect redu...

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Abstract

The invention discloses a preparation method of compound polysaccharide consisting of schizophyllum commune exopolysaccharide and oat polysaccharide. The method comprises the following steps of: inoculating schizophyllum commune on a PDA (potato dextrose agar) plating medium to perform rejuvenation and activation cultivation to obtain a plating strain; inoculating the plating strain to a seed medium, and culturing to obtain a primary strain; inoculating the primary strain in the seed medium, and culturing to obtain a secondary strain; inoculating the secondary strain on a basic medium and fermenting to obtain fermented mash; and extracting and purifying the fermented mash to obtain the compound polysaccharide, wherein the seed medium comprises the following components in 1 kilogram of seed medium: 50g of glucose, 5g of monopotassium phosphate, 2.4g of magnesium sulfate, 3g of yeast extract, and the balance of tap water; and the asic medium comprises the following components in 1 kilogram of basic medium: 50g of glucose, 5g of monopotassium phosphate, 2.4g of magnesium sulfate, 4.5g of beef extract, 3g of calcium carbonate, 5g of oat bran and the balance of tap water.

Description

technical field [0001] The invention relates to a preparation method of a composite polysaccharide, in particular to a preparation method of a composite polysaccharide composed of schizophyllum exopolysaccharide and oat polysaccharide. Background technique [0002] In recent years, with the in-depth research on polysaccharides, it has been found that polysaccharides have quite a lot of physiological functions, such as immune regulation, anti-oxidation, anti-tumor, anti-coagulant activity, lowering blood sugar, lowering blood lipids, and anti-hypoxia. [0003] Polysaccharides come from a wide range of sources, mostly from animals, plants, microorganisms and seaweeds. Different polysaccharides have different biological activities, but in general, fungal polysaccharides have higher anti-tumor activity, and higher plant polysaccharides have better immune-promoting effects. Therefore, fungal polysaccharides and plant polysaccharides have been paid more and more attention in rece...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 徐宝军
Owner 徐宝军
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