Reagent device for detecting anti-Sc170 antibody and method thereof

Abstract

The invention discloses a reagent device for detecting an anti-Sc170 antibody and a method thereof; the reagent device is long strip-shaped, comprises a substrate with eight hole sites and a handle disposed at one end of the substrate; the eight hole sites comprise a sample hole, an adjuvant hole, an enzyme conjugate hole, a substrate hole, a stopping solution hole, a diluent hole, a reaction hole, and a dilution hole, which are arranged in sequence from the end near the handle. The reagent device of the invention realizes anti-Sc170 antibody detection with the device based on a principle of enzyme-linked immunoassay, is an independent, single-sample analytic detection method, and can be used cooperatively with corresponding specific analytical instruments; during detection, a detection reagent or a sample is injected by using a full automatic precision liquid filling device, which provides advantages of operation automation, accurate filling amount, high accuracy and precision of detection results; and the reagent device has wide application prospects.

Description

technical field [0001] The application of the present invention relates to a reagent device and a method for detecting anti-Scl70 antibodies, which belong to the technical field of clinical immunology detection. Background technique [0002] Systemic sclerosis (SSc) is a connective tissue disease characterized by hardening of collagen fibers in various systems of the skin. Systemic scleroderma that affects the internal organs is also called systemic sclerosis. The disease is second only to lupus erythematosus among connective tissue diseases. Most patients are female, and the ratio of female to male is about 3:1. The age of onset is more common between 20 and 50 years old. [0003] Scl 70 is a ribozyme composed of 765 amino acids, which regulates and catalyzes the conversion of DNA topology by transiently breaking single-strand DNA, and is used to unwind DNA supercoils to enable cellular functions. Anti-Scl 70 antibodies bind to one or more of the 6 epitopes in the enzym...

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Problems solved by technology

[0007] (1) Only qualitative and semi-quantitative analysis can be carried out, and the specific amount of the tested substance cannot be obtained

[0008] (2) The operation steps are cumbersome and the test takes a long time

[0009] (3) The detection sensitivity needs to be improved

[0012] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results;

[0013] (2) The operation is relatively complicated and requires expensive fluorescence microscopes, which are difficult to promote in many primary hospitals and are not suitable for laboratories with a large number of specimens;

[0014] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination;

[0015] (4) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient;

[0016] (5) Only qualitative detection can be carried out, and quantitative measurement cannot be carried out

[0019] (1) Use 12×8 type, 6×8 type, 8×12 type or whole plate type 96-well special microwell plate as the antigen coating equipment and reaction container, which can only be divided into 12 batches and 6 batches when used , 8 batches or the whole board can be used at one time, and independent and single-person testing cannot be carried out;

[0020] (2) There are many kinds of reagents used in quantitative determination, and each detection reagent must be contained in a reagent...

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