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Resonance scattering spectral detection method for tetracycline based on nano-gold catalysis

A technique of resonance scattering and tetracycline, applied in the field of tetracycline, can solve the problem of unsatisfactory detection line, and achieve the effects of low price, simple operation and good selectivity

Active Publication Date: 2013-07-10
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention proposes a method for detecting tetracycline based on nano-gold catalysis by resonant scattering spectroscopy, which overcomes defects such as the need for modification of nucleic acid aptamers and unsatisfactory detection lines, and provides a sensitive, selective, and Inexpensive and efficient tetracycline detection method

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  • Resonance scattering spectral detection method for tetracycline based on nano-gold catalysis
  • Resonance scattering spectral detection method for tetracycline based on nano-gold catalysis
  • Resonance scattering spectral detection method for tetracycline based on nano-gold catalysis

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Embodiment 1

[0029] Such as figure 1 As shown, this embodiment includes the following steps:

[0030] 1) Prepare a tetracycline detection system with known concentration: take 11 centrifuge tubes containing a mixture of tetracycline nucleic acid aptamer and nano-gold, and incubate at 25°C for 15 minutes; The tetracycline content in the detection system is maintained at 0.0095-2.5 μmol / L, and then the centrifuge tube is incubated at 25°C for 15 minutes after thorough mixing; add 7 μL of 500 mmol / L NaCl solution to the above mixture, mix well and make to volume To 500μL, reserved for the following determination.

[0031] 2) Take a centrifuge tube and replace the tetracycline standard solution with 5 μL of ultrapure water, and treat the others according to step 1 as the blank control system solution.

[0032] 3) Preparation of Fehling's reagent-glucose reduction system: Take 11 test tubes and add 10 μL of 0.028mol / LCuSO to each test tube 4 , 100μL 6.25mol / L NaOH, 200μL 1.23mol / L KNaC 4 h ...

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Abstract

The invention provides a resonance scattering spectral detection method for tetracycline based on nano-gold catalysis. The method comprises the following steps: constructing detection systems containing tetracycline with known concentration; carrying out resonance scattering spectral signal scanning by using a fluorophotometer so as to obtain a resonance scattering signal variable standard curve; respectively adding a to-be-detected object and ultrapure water, i.e., a control sample, into a mixed liquor containing a tetracycline aptamer and nano-gold, and adding a sodium chloride solution for an agglomeration reaction; and carrying out mixing with a solution containing copper sulfate, sodium hydroxide and potassium sodium tartrate and carrying out resonance scattering spectral signal scanning by using the fluorophotometer to obtain a resonance scattering signal variable curve so as to obtain the concentration of tetracycline in the to-be-detected object. The detection method provided by the invention overcomes the defects that a nucleic acid aptamer needs modification, a detection line is non-ideal and the like and has the advantages of sensitivity, good selectivity, low cost and high efficiency.

Description

technical field [0001] The present invention relates to a method in the technical field of food safety, in particular to a method of using gold nanometer wrapped by aptamers to catalyze and produce cuprous oxide enhanced resonance scattering spectroscopy to detect the concentration of tetracycline in milk in the range of 0.0095-2.5 μmol / L, The method with a minimum detection limit of 9.5 nmol / L tetracycline. Background technique [0002] Tetracycline is a common antibiotic drug, but with the increase of its usage, its residue in animal food and environment has aroused widespread concern. Tetracycline residues in the environment can enhance microbial resistance and even the growth of superbugs, and can also accumulate in animals and plants through the food chain; the hazards of tetracycline residues in animal foods are chronic toxicity hazards, but long-term intake may lead to various Pathological changes in various organs and even some allergic constitutions can cause aller...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 周培罗艳芳贺兰智文婷吴远根詹深山刘乐
Owner SHANGHAI JIAO TONG UNIV
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