Application of ethanol to regulating and controlling mytilus coruscus larva metamorphosis

A technology for mussel larvae and larvae, which is applied to the field of ethanol in the regulation of the metamorphosis of mussel larvae, achieving the effects of low cost, stable action and high induction efficiency

Inactive Publication Date: 2013-07-24
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the relationship between ethanol and the regulation of metamorphosis in mussel larvae

Method used

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  • Application of ethanol to regulating and controlling mytilus coruscus larva metamorphosis
  • Application of ethanol to regulating and controlling mytilus coruscus larva metamorphosis

Examples

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Embodiment 1

[0022] 1 Experimental materials

[0023] Sexually mature mussels were collected from Lycium barbarum sea area, Shengsi County, Zhejiang; ethanol reagent was purchased from Sinopharm Chemical Reagent Co., Ltd.

[0024] 2 Experimental methods

[0025] 2.1 Cultivation of eye-spot larvae of thick-shelled mussel

[0026] Collect the sexually mature individuals of the thick-shelled mussels, brush off the sludge and barnacles attached to the body surface of the thick-shelled mussels with tools, and rinse them with sea water. After drying in the shade for 24 h, transfer to a 10 L polycarbonate container filled with filtered seawater (GF / C: 1.2 μm, FSW: filtered seawater) at 20 °C. Adults begin to release sperm and eggs, which are stirred to fertilize them. Fertilized eggs were placed in an incubator at 18°C ​​for 2 days in the dark. After 2 days, fertilized eggs develop into D-shaped disc larvae, and the larvae are cultivated in a beaker filled with 2 L of filtered seawater, a...

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Abstract

The invention relates to an application of ethanol to accelerating mytilus coruscus larva metamorphosis and/or shortening spatfall time of mytilus coruscus larvae, and further relates to a method of improving a mytilus coruscus larva metamorphosis rate or shortening a mytilus coruscus cultivating period. The method comprises the following steps of putting the mytilus coruscus larvae into an ethanol solution with the concentration of 0.1-1 M to carry out exposure treatment for 1-24 hours; and then transferring into a utensil containing seawater to cultivate. According to the application and the method disclosed by the invention, the ethanol can obviously improve the mytilus coruscus larva metamorphosis rate and shorten the mytilus coruscus cultivating period and the cost of the ethanol is low, so that the ethanol has a wide application prospect in a mytilus coruscus cultivation industry; and the application further provides suitable dosage, treating time and induction time when the ethanol is used as an inductor to improve the mytilus coruscus larva metamorphosis rate; and under the condition, the induction efficiency is high, the effect is stable and specifications of obtained young shellfishes are the same, so as to provide guidance to production practices.

Description

technical field [0001] The invention relates to a new application of ethanol, in particular to the application of ethanol in the regulation of metamorphosis of mussel larvae. Background technique [0002] thick-shelled mussels ( Mytilus coruscus ), Molluscs / Bivalvia, distributed in my country's Yellow Sea, Bohai Sea and East China Sea coasts and Taiwan and other places. Thick-shelled mussels live by attaching to the appendages with silk, and use gills to filter food. Choose to eat tiny organisms and organic debris in seawater. Its breeding season is twice a year and is one of the fastest growing species of marine shellfish. The developmental stages of mussels include fertilized egg, mulberry stage, blastula stage, trochophore larvae, D-shaped larvae, shell top larvae, creeping larvae, etc. There is a planktonic larval stage before becoming an attached adult, and the larvae attach to Substratum, metamorphosis becomes spat. [0003] In the current artificial breeding proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨金龙李树恒梁箫李家乐
Owner SHANGHAI OCEAN UNIV
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