Usage of potassium ions in controlling metamorphosis of mytilus coruscus larvae

A technology of thick-shelled mussels and potassium ions, which is applied in the field of chemical ions potassium ions, to achieve the effects of shortening the time of attaching seedlings, increasing the metamorphosis rate, and cheap prices

Inactive Publication Date: 2013-07-24
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Usage of potassium ions in controlling metamorphosis of mytilus coruscus larvae
  • Usage of potassium ions in controlling metamorphosis of mytilus coruscus larvae
  • Usage of potassium ions in controlling metamorphosis of mytilus coruscus larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 Experimental materials

[0029] Sexually mature mussels were collected from Lycium barbarum, Shengsi County, Zhejiang; potassium chloride, potassium sulfate, and tetraethylamine were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0030] 2 Experimental methods

[0031] 2.1 Cultivation of eye-spot larvae of thick-shelled mussel

[0032] Collect the sexually mature individuals of the thick-shelled mussels, brush off the sludge and barnacles attached to the body surface of the thick-shelled mussels with tools, and rinse them with sea water. After drying in the shade for 24 h, transfer to a 10 L polycarbonate container filled with filtered seawater (GF / C: 1.2 μm filter membrane, FSW: filtered seawater) at 20 °C. Adults begin to release sperm and eggs, which are stirred to fertilize. Fertilized eggs were placed in an incubator at 18°C ​​for 2 days in the dark. After 2 days, the fertilized eggs develop into D-shaped larvae, and the larvae are cultivated in a beake...

Embodiment 2

[0050] This embodiment tests the influence of potassium ion solution on the metamorphosis rate and mortality of larvae when the potassium ion solution is exposed to 1h, 8h, and 16h respectively at a concentration of 50 mM, and the effect of tetraethylamine solution on 1 mM concentration. The effect on the metamorphosis rate and mortality of the larvae when the larvae were treated for 30 minutes, 45 minutes and 60 minutes, the specific operation was the same as in Example 1.

[0051] The potassium ion solution was exposed to 50 mM concentration of mussel larvae for 1 h, 8 h, and 16 h, respectively, and then transferred to filtered seawater for cultivation. The results of larval metamorphosis and mortality after 96 h are shown in Table 1 and Table 2.

[0052] Table 1 Metamorphosis rate of larvae after 96 h incubation under different treatment conditions of potassium ion solution

[0053]

[0054] Table 2 Mortality of larvae incubated for 96 h under different treatment conditi...

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Abstract

The invention relates to a usage of potassium ions in promoting the metamorphosis of mytilus coruscus larvae and shortening the seed settlement time of the mytilus coruscus larvae and further relates to a usage of a potassium ion antagonist in inhibiting the metamorphosis of the mytilus coruscus larvae and a method for promoting or inhibiting the metamorphosis of the mytilus coruscus larvae. The usage and the method have the advantages that evidences prove that by the potassium ions, the metamorphosis rate of the mytilus coruscus larvae can be improved, and the seed settlement time of the mytilus coruscus larvae can be shortened, so that the potassium ions can be used for increasing the production success rate of mytilus coruscus seeds and shortening the production period; and meanwhile, cheap and nontoxic sylvite is suitable for large-scale seed production. The evidences also prove that the potassium ion antagonist can inhibit the metamorphosis of the mytilus coruscus larvae, and a novel way is provided to control marine contamination. The conclusion of the study is helpful in stating the settlement and metamorphosis mechanism of mytilus coruscus.

Description

technical field [0001] The invention relates to a new application of the chemical ion potassium ion, in particular to the application of the potassium ion in the regulation and control of metamorphosis of mussel larvae. Background technique [0002] thick-shelled mussels ( Mytilus coruscus ), Molluscs / Bivalvia, distributed in my country's Yellow Sea, Bohai Sea and East China Sea coasts and Taiwan and other places. Thick-shelled mussels live by attaching to the appendages with silk, and use gills to filter food. Choose to eat tiny organisms and organic debris in seawater. Its breeding season is twice a year and is one of the fastest growing species of marine shellfish. The developmental stages of thick-shelled mussels include fertilized egg, mulberry stage, blastula stage, trochophore larvae, D-shaped larvae, shell top larvae, creeping larvae, etc. There is a planktonic larval stage before becoming an attached adult, and the larvae attach to Substratum, metamorphosis beco...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨金龙李树恒李无霜梁箫李家乐
Owner SHANGHAI OCEAN UNIV
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