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Method of evaluating security of transgenic plants

A genetically modified plant, safe technology, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as human health hazards

Inactive Publication Date: 2013-08-21
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology

However, the application of genetically modified crops in agricultural production is still controversial. The focus of controversy is whether the genetically modified crops will bring unexpected effects and cause harm to human health. In this case, the safety assessment of genetically modified crops is particularly important important
[0003] The current means of assessing the safety of genetically modified crops is mainly the analysis of targeted components, which has certain limitations and can only detect a limited amount of molecules
At present, the urgent problem to be solved is to objectively and comprehensively evaluate the impact of transgene on the stability of molecular characteristics of crops and the degree of influence, which will be related to the industrialization of transgenic crops and social concerns about the safety of transgenic crops

Method used

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  • Method of evaluating security of transgenic plants
  • Method of evaluating security of transgenic plants

Examples

Experimental program
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Embodiment 1

[0027] Example 1, Molecular Characteristic Detection Technology and Technical Standards of Genetically Modified Crops

[0028] 1. Selection of experimental materials

[0029] Transgenic rice 2036-1a: Transgenic rice transformed with the insect resistance gene cry1Ac / sck (progeny with stable traits); the public can obtain it from the Institute of Botany, Chinese Academy of Sciences; references mentioning this plant material: Liu Yufang et al., 2007, Chinese Agricultural Sciences , 40: 1181-1189.

[0030] Minghui 86 (MH86): parental control (indica rice) of transgenic rice 2036-1a; the public can obtain it from the Institute of Botany, Chinese Academy of Sciences; references mentioning this plant material: Zhang Jianxin et al., 2000, Journal of Jiangxi Agricultural University, 22: 485-490.

[0031] Transgenic rice Bar68-1: Transgenic rice transformed with the herbicide-resistant gene bar; the public can obtain it from the Institute of Botany, Chinese Academy of Sciences; refer...

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Abstract

The invention discloses a method of evaluating security of transgenic plants. The method comprises: (1) extracting total proteins of to-be-detected samples, performing 2D-DIGE electrophoresis and fluorescence image analysis, thereby obtaining proteome maps of the to-be-detected samples, wherein the to-be-detected samples comprises samples 1, 2, 3 and 4, the sample 1 is a to-be-detected transgenic plant, the sample 2 is a parent plant of the to-be-detected transgenic plant, the sample 3 and the sample 2 are different plants of a same cultivated species, and the sample 4 and the sample 2 are different cultivated species; (2) selecting proteins appearing in more than 70 % of proteome maps for principal component analysis; and (3) determining according to results of the principal component analysis. The method provided by the invention has practical application values for systematic assessment of molecular characteristics and biological safety of genetically modified crops.

Description

technical field [0001] The invention relates to a method for evaluating the safety of transgenic plants. Background technique [0002] Rice is one of the most important food crops in the world and the largest food crop in my country. With the deepening of molecular genetics research and the improvement of transgenic technology, transgenic technology has become the most important technical means to improve the agronomic traits of rice and other crops. However, the application of genetically modified crops in agricultural production is still controversial. The focus of controversy is whether the genetically modified crops will bring unexpected effects and cause harm to human health. In this case, the safety assessment of genetically modified crops is particularly important important. [0003] The current means of assessing the safety of genetically modified crops is mainly the analysis of targeted components, which has certain limitations and can only detect a limited amount...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/447G01N27/62
Inventor 王台巩春燕李琦
Owner INST OF BOTANY CHINESE ACAD OF SCI
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