Large-scale cultivation method for Guangdong fruiting bodies of cordyceps military

Abstract

The invention relates to the technical field of cultivation of fruiting bodies of cordyceps military, in particular to a large-scale cultivation method for Guangdong fruiting bodies of cordyceps military. The large-scale cultivation method includes the steps: A, distribution; B, preparation of strains including B1, slope strains, B2, shake flask strains and B3 fermented strains; C, inoculation and mycelium culture; D, fruiting body culture; and E, recovery. The deep fermented strains replace existing multistage shake flask strains and are high in hyphae vigor, mycelium pellets are fine in size uniformity, inoculation can be performed by an automatic inoculation machine or manual spraying in a sterile environment, assembly line operation is realized, inoculation amount is stable, each bottle is 40-60mL, spraying is uniform, hyphae rapidly sprout, contamination rate is reduced, fruiting body coming time is 3-5 days shorter than that of the prior art, the whole production cycle is shortened by about 10 days, and labor and raw material costs can be saved as compared with shake flask strain inoculation in the prior art.

Description

technical field [0001] The invention relates to the technical field of cordyceps fruiting body cultivation, in particular to a large-scale cultivation method for the fruiting body of Cordyceps guangdong. Background technique [0002] More than 450 species of Cordyceps fungi have been discovered in the world, more than 100 species have been reported in China, and more than 30 species have been found in Guangdong. Some data show that many fungi of the genus Cordyceps are rich in various amino acids, trace elements, vitamins and other nutrients, as well as active ingredients such as adenosine, cordycepin, and cordycepic acid, which have anti-tumor, anti-pathogen, anti-oxidation and enhance human immunity. and other pharmacological functions, with the improvement of people's living standards and the increase in demand for healthy food, Cordyceps sinensis is increasingly loved by people, but so far, Cordyceps sinensis has not yet been artificially cultivated on a large scale, and...

AI Technical Summary

Problems solved by technology

[0011] The reference document discloses a Guangdong variant strain of Cordyceps japonica, which provides materials for the subsequent research and development of the Guangdong variant strain of Cordyceps japonica. The cultivation methods disclosed in the reference document include: production of mother species → production of first-level liquid strains → production of secondary production species → Inoculation and weeding management → harvesting, the cultivation method of the comparison file has the following shortcomings: multi-stage shake flask species are used for inoculation, the mycelium vitality of multi-stage shake flask species is weak, and the size uniformity of mycelium balls is poor, which is difficult to achieve Large-scale automated cultivation; and mycelium germination is slow, and the time for fruiting bodies is slow, resulting in the extension of the entire production cycle and a waste of manpower and raw materials