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Imaging chamber for supporting multiple investigation of cells and tissues by various techniques

An imaging and cell technology, applied in laboratory equipment, laboratory containers, chemical instruments and methods, etc., can solve the problem of single use

Inactive Publication Date: 2013-09-11
LACERTA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typically, these imaging chambers are used with only one imaging instrument, such as a light microscope or confocal microscope, for a single purpose

Method used

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  • Imaging chamber for supporting multiple investigation of cells and tissues by various techniques
  • Imaging chamber for supporting multiple investigation of cells and tissues by various techniques
  • Imaging chamber for supporting multiple investigation of cells and tissues by various techniques

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Embodiment Construction

[0021] The following description illustrates numerous specific details in order to provide a thorough understanding of the embodiments. However, these embodiments can be practiced without one or more of these specific details and still fall within the scope of protection claimed in the appended claims.

[0022] figure 1 and 2 Two main housing parts are shown: vessel 1 and lid 2 . The upper housing part - cover 2 is made of a completely transparent (aqua-clear) polymer in order to allow studies through a transmitted light microscope. Openings 3 , 4 of different sizes are provided in the cover 2 . The smaller opening 3 clasps an annular silicon insert 5 provided in the lid 2 for ventilation by means of an extension 6 inserted into this opening. The larger opening 4 allows ventilation through the insert 5 and provides a connection surface for the perfusion tube. Around the circumference of the lid 2 there is provided a wall 7 which surrounds the upper edge 8 of the vessel 1 ...

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Abstract

The imaging chamber according to the invention comprises a housing with a lower part and an upper part, wherein said lower part is a dish (1) and said upper part is a lid (2) provided with a skirt (9) surrounding the upper rim (10) of the dish (1), the bottom of the dish (1) is provided with a cover slip (12) to be used in confocal microscopy and at least a part of the lid (2) is transparent wherein the lid (2) is provided with a breathing silicon insert (5). The silicon insert (5) comprises a circular ring part fixed to the inner surface of the lid (2) and a skirt (14 ) parallel with the upper rim (10) of the dish (1) and supported by the skirt (9) of the lid (2) and provided with an annular groove (15 ) tight fitted on the upper rim (10) of the dish (1); and the lid (2) is provided with openings (4) to allow breathing of the housing.

Description

technical field [0001] The present invention relates to an imaging chamber for expanding cells under standard cell culture conditions and / or for imaging via various imaging techniques such as optical microscopy, confocal microscopy, microelectron computed tomography ( CT) and nanometer single photon emission computed tomography (SPECT) / CT for long-term studies of cultured cells or tissues. Background technique [0002] Over the years, many custom live-cell imaging chambers have been designed to meet specific needs, and a large number of commercial perfusion and imaging chambers also exist today. These imaging chambers include glass-bottomed Petri dishes, multi-well chambers mounted on microscope slides, heated stages with multiple interchangeable perfusion adapters, and special imaging chambers with conductive coatings for tight temperature control . [0003] US 4,839,046 (Joseph A. Chandler) discloses a bioreactor that supplies a fresh oxygenated fluid nutrient base into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/22B65D43/02
CPCB01L3/508B01L2300/048C12M23/10C12M23/22C12M23/38C12Q1/02
Inventor 维克多·埃扎皮萨姆博·洛曹欧顿·瓦格耐尔米克洛斯·韦斯尔
Owner LACERTA TECH