Application of rice protein CHR1 in adjusting content of plant nitrate

A nitrate content, nitrate technology, applied in the direction of application, plant products, botany equipment and methods, etc., can solve the problems of increasing agricultural production costs, environmental pollution, etc.

Inactive Publication Date: 2013-09-18
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive use of nitrogen fertilizer not only greatly increases the cost of agricultural production, but also a large amount of chemica...

Method used

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  • Application of rice protein CHR1 in adjusting content of plant nitrate
  • Application of rice protein CHR1 in adjusting content of plant nitrate
  • Application of rice protein CHR1 in adjusting content of plant nitrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, rice protein CHR1 is a nitrate transporter

[0031] 1. Screening of rice mutants

[0032] Studies have shown that chlorate, as a toxic analog of nitrate, can be used to identify Arabidopsis mutants with nitrate uptake defects (literature: Tsay YF, Schroeder JI, Feldmann KA, & Crawford NM (1993) The Herbicide Sensitivity Gene Chl1of Arabidopsis Encodes a Nitrate-Inducible Nitrate Transporter. Cell72(5):705-713.Wilkinson JQ& Crawford NM(1991) Identification of the Arabidopsis CHL3Gene as the Nitrate Reductase Structural Gene NIA2.Plant Cell3(5):461-47 .). In this experiment, chlorate was used to screen the rice T-DNA mutant library created by the inventor's research team using rice Zhonghua 11 as the background material, and a rice mutant chr1 with significant resistance to chlorate was obtained.

[0033] The method and results of the above-mentioned chlorate screening test are as follows:

[0034]The mutant seeds and wild-type rice Zhonghua 11 were subjecte...

Embodiment 2

[0065] Example 2, overexpression of protein CHR1 increases the nitrate content of rice or improves the utilization rate of rice to nitrate

[0066] 1. Construction of plant recombinant expression vector

[0067] Use primer 5'- CCCGGG CGCCGTCTTACTCTCTCTG-3' (the slash part of the base is the XmaI restriction recognition sequence, and the rest of the sequence is the same as the 44th-62nd position of the sequence listing sequence 2) and 5'- TCTAGA AATTATACCCGGCGAAATAC-3' (the slashed base is the recognition sequence for XbaI digestion, and the rest of the sequence is reverse complementary to the 2068-2087 position of sequence 2 in the sequence listing), the cDNA obtained by reverse transcription of the total RNA of rice Zhonghua 11 is Template, carry out PCR amplification, recover and purify the fragment of 1.8kb, after double digestion with XmaI and XbaI, connect with the carrier backbone of the vector pCAMBIA2300-Actin1 after double digestion with XmaI and XbaI, it is confir...

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Abstract

The invention discloses application of rice protein CHR1 in adjusting content of plant nitrate. The protein CHR1 is the protein as shown in sequence 1 of a sequence table, and can be used for adjusting the content of the plant nitrate or adjusting the utilization rate of the plant to the nitrate, or adjusting the substance of the protein expression quantity as shown in the sequence 1 of the sequence tale or adjusting the content of the plant nitrate or adjusting the utilization rate of the plant to the nitrate. According to the experiments, the protein CHR1 as shown in the sequence 1 of the sequence table is nitrate transport protein which is over-expressed in a gene rice strain of the protein CHR1; with the increase of the relative expression amount of the protein CHR1 coding gene, the nitrate content or the utilization rate to the nitrate is also obviously improved. According to the results, the protein CHR1 has important application potential in improving the utilization of the plate to the nitrate. The invention provides a way for culturing a crop with a high-nitrogen fertilizer utilization rate.

Description

technical field [0001] The invention relates to the application of rice protein CHR1 in regulating the nitrate content of plants. Background technique [0002] Among the essential mineral nutrients for plants, nitrogen is the most required element. In order to promote crop yields, the amount of nitrogen fertilizer applied in agriculture is increasing, which leads to a sharp decrease in the nitrogen use efficiency (NUE) of crops, and the nitrogen fertilizer required to produce a unit of food is constantly increasing. Excessive use of nitrogen fertilizer not only greatly increases the cost of agricultural production, but also a large amount of chemical fertilizer residues also leads to serious environmental pollution, such as soil acidification and compaction and water eutrophication. Therefore, improving the nitrogen use efficiency of crops and reducing the use of nitrogen fertilizer will be the key to solving these serious problems. Nitrate is the most important form of ni...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 储成才胡斌王威车荣会刘林川王义琴梁成真
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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