Hongyan strawberry nitrate transporter gene FaNRT1.1 and application thereof

A technology for nitrate transport and red-faced strawberries, applied in the fields of application, genetic engineering, and plant genetic improvement, to achieve the effects of promoting fruit ripening, improving strawberry quality and yield, and promoting absorption

Pending Publication Date: 2022-05-31
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of how to study strawberry nitrate transporter related genes and their expression and corresponding models, and provide the red-faced strawberry nitrate transporter gene FaNRT1.1 and its application

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  • Hongyan strawberry nitrate transporter gene FaNRT1.1 and application thereof
  • Hongyan strawberry nitrate transporter gene FaNRT1.1 and application thereof
  • Hongyan strawberry nitrate transporter gene FaNRT1.1 and application thereof

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Embodiment Construction

[0022] The above and other technical features and advantages of the present invention will be described in more detail below with reference to the accompanying drawings.

[0023] 1. Cloning and analysis of the gene and promoter of Nitrate transporter gene FaNRT1.1 in strawberry.

[0024] The present invention utilizes software FPCR and BioXM2.6 to design specific primers by comparing the octoploid strawberry genome data according to the transcriptome data of red-colored strawberry and NCBI. Using N11F and N11R as primers and the cDNA of strawberry root as template, the NRT1.1 gene was amplified by LATaq enzyme (Takara) system.

[0025] The PCR amplification products were detected by 1% agarose gel electrophoresis, and the gel pieces of the target length bands were cut out, and the target fragments were recovered by using the TIANgelMidiPurification Kit. The target fragment recovered from the gel was ligated with pMDTM19-T vector, transformed into E. coli DH5α competent cells,...

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Abstract

The invention relates to the technical field of plant genes, in particular to a Hongyan strawberry nitrate transporter gene FaNRT1.1 and application thereof.The nucleotide sequence of the Hongyan strawberry nitrate transporter gene FaNRT1.1 is shown as SEQ ID NO.1. The Hongyan strawberry nitrate transporter gene FaNRT1.1 is obtained by cloning from Hongyan strawberries, and the nucleotide sequence of the Hongyan strawberry nitrate transporter gene FaNRT1.1 is shown as SEQ ID NO.2. The Hongyan strawberry nitrate transporter gene FaNRT1.1 is shown as SEQ ID NO.1. The expression mode and response modes to different nitrogen forms, nitrate nitrogen, hormones and other abiotic stress are studied, so that the gene is applied to screening germplasm materials, cultivating high-quality varieties and coping with abiotic stress according to different response states.

Description

technical field [0001] The invention relates to the technical field of plant genes, in particular to the Nitrate transporter gene FaNRT1.1 of rhododendron and application thereof. Background technique [0002] NRT1.1 is the only confirmed dual-affinity nitrate transporter and putative nitrate receptor. Arabidopsis AtNRT1.1 is the first nitrate transporter in the NRT1 family to be discovered and the function of which is most clearly studied. In 1993, Tsay obtained the first mutant of the plant nitrate transport protein gene CHL1 (AtNRT1.1) by screening the nitrate analog chlorate for the first time; the gene was cloned and heterologous in Xenopus oocytes Expression, the study found that this gene is a low-affinity transporter gene encoding nitrate-induced, and the expression increased when the cell was low pH, while the expression decreased when the ammonium salt was treated. CHL1 also has the functional characteristics of a high-affinity nitrate transport protein. Subseque...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/08A01H6/74
CPCC07K14/415C12N15/8261C12N15/8271
Inventor 方从兵孙启涵郝福玲吴沐谦吴思鑫
Owner ANHUI AGRICULTURAL UNIVERSITY
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