Rapidly breeding method for ampelocalamus luodianensis

The invention is a fast technology for Pengzhu, which is applied in the field of establishing a rapid propagation system of Pseudomonas serrata by using tissue culture technology. The effect of low transportation cost and long breeding cycle

Active Publication Date: 2014-12-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: aiming at the deficiencies in the prior art, the purpose of the invention is to provide a method for the rapid propagation of Bambusa, to overcome the difficulties in the reproduction of Bambusa, alleviate the problem of sharp reduction in the population of Bambusa in the natural state, and to fill the gap Blanks in the Rapid Propagation of Bamboo

Method used

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  • Rapidly breeding method for ampelocalamus luodianensis
  • Rapidly breeding method for ampelocalamus luodianensis
  • Rapidly breeding method for ampelocalamus luodianensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 External disinfection of small penguins

[0033] Semi-lignified branches (i.e. explants: plants that grow robustly and are free from diseases and insect pests, take semi-lignified branches with ungerminated stalk buds or just germinated stalk buds, stalk buds wrapped in leaf sheaths) collected outdoors After indoors, cut into small sections, each with a stalk bud, leave 0.5-1cm above and below each section, take 2-3ml of white cat detergent, dissolve it in 2-3L of tap water, and place the explants in Soak it in detergent water (concentration about 1‰) for 2 minutes, and shake it constantly, then rinse it under running water for 2-3 hours, and dry the surface with filter paper. After pretreatment, the stems were placed on the ultra-clean workbench and washed with 70% ethanol and 0.1% HgCl 2 The explants were sterilized and sterilized by the solution, rinsed with sterile water 3 times after ethanol sterilized treatment, and 5 times with sterilized water after m...

Embodiment 2

[0040] Example 2 Induction of Bamboo Cluster Buds

[0041] In this experiment, MS was selected as the basic medium, and the combination of growth regulators 6-BA, NAA and KT was used to induce the production of cluster buds, and the explants treated by the best disinfection method (Example 1) selected by the test were used as test materials To induce cluster buds, select the most suitable hormone ratio for cluster bud induction. 24 bottles were combined for each concentration, and 3 explants were inoculated in each bottle. 25±1°C, light 3000 lx, 14h / d, conduct induction culture; observe once every 5 days, the observation period is 25 days, count the number of induced cluster buds, observe and record the growth of buds.

[0042] Stem buds of explants, such as figure 1 Shown; stalk bud germination, such as figure 2 shown; cluster bud induction, such as image 3 shown. The experimental results are shown in Table 2, and the optimal formula for the combination of hormone rati...

Embodiment 3

[0045] Example 3 Proliferation of Buds of Bamboo Bambusa

[0046] Cut the induced cluster buds into small pieces with several buds (3-5), transfer them to the proliferation medium after separation, and carry out proliferation culture at 25±1°C, light 3000 lx, 14h / d . The combination of growth regulators 6-BA, KT and NAA was used for proliferation. The experiment adopted three-factor and three-level orthogonal design to study the effects of different concentration combinations on subculture.

[0047] Cluster buds proliferate as Figure 4 shown. The experimental results are shown in Table 3. The optimal formula for the combination of proliferating hormones in clustered buds is A 3 B 2 C 1 , namely: MS+6-BA 5mg / L+KT 0.5mg / L. Comparing the experimental data, it can be seen that the range of 6-BA is 1.04, the range of KT is 0.26, and the range of NAA is 0.47. The effect of each hormone type on the multiplication of clustered buds is: 6-BA>NAA>KT .

[0048] Table 3 Experimen...

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Abstract

The invention discloses a rapidly breeding method for ampelocalamus luodianensis, comprising: explant selection of the ampelocalamus luodianensis, explant disinfection of the ampelocalamus luodianensis, multiple shoot induction of the ampelocalamus luodianensis, multiple shoot propagation of the ampelocalamus luodianensis, multiple shoot rhizogenesis of the ampelocalamus luodianensis, and tissue culture plant transplanting of the ampelocalamus luodianensis. The rapidly breeding method for ampelocalamus luodianensis has advantages of small investment, fast seedling growing speed of the ampelocalamus luodianensis, high survival rate and consistent growth vigor of the sprouts, and overcomes various disadvantages of traditional mother bamboo transplanting, such as low breeding efficiency, large damage of ecological environment, long breeding period and high transport cost. As the rapidly breeding method for ampelocalamus luodianensis, the breeding of ampelocalamus luodianensis by the method is not limited by seasons, transport cost is low, and the method is suitable for industrial seedling growing production, and provides a rapid and effective approach for rapidly recovering amount of the ampelocalamus luodianensis in a natural state and performing ecological benefits and economic benefits of the ampelocalamus luodianensis in karst areas.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of bamboo plants, and in particular relates to a method for establishing a rapid propagation system of bamboo by tissue culture Background technique [0002] Ampelocalamus luodianensis is a bamboo species of the genus Ampelocalamus in the Poaceae Bamboo subfamily. It is an endangered plant in karst areas. At present, it is mainly distributed in Luodian County, Changshun County, Ziyun Miao and Buyi Autonomous County, Guizhou Province. Wangmo County. The entire distribution area is located at north latitude: 24°58′-26°03′; east longitude: 105°25′-107°03′, and the vertical distribution height is between 650-1250m. Its appearance is beautiful, and it has the value of cultivation and ornamental. At the same time, the small bamboo can grow well in the karst area with harsh environment, and it can tolerate drought and barrenness. The interception of the forest is significan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭婷婷刘国华丁雨龙王福升林树燕汪玉凤
Owner NANJING FORESTRY UNIV
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