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Seedling raising method of plateau rhodiola crenulata

A technology of Rhodiola grandiflorum and seeds, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long breeding cycle and inapplicability, achieve fast breeding speed, increase germination rate, and good heredity Effect

Active Publication Date: 2012-07-04
TIBET ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter has better heredity, but the reproductive cycle is longer
And the existing seedling raising methods of Rhodiola grandis are not suitable for high-altitude areas like Tibet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take the seedlings cultivated in test tubes as the source of seedlings, that is, the seedlings obtained by inoculating seeds in test tubes, as the basic seedlings: after soaking the seeds for 12-24 hours, soak the materials in 70% ethanol for 30 seconds on the ultra-clean workbench, Soak with 0.1% HgCl for 25-30min, then rinse with sterilized sterile water for 3-5 times, and then dry the water on the surface of the explant with sterile filter paper for use. The obtained sterile material is inoculated on the Ms medium under sterile conditions, and the seeds germinate in about 7 days, and then transferred to 1 / 2 Ms medium to simulate the wild environment and control the temperature difference between day and night. The daily temperature is 18°C-22°C, The night temperature is 4°C-6°C, the temperature difference should be greater than or equal to 12°C, and the light intensity is about 1500-2000LX. Combined with natural light, the light time is controlled at 10-11 hours to in...

Embodiment 2

[0030]A seedling raising method of Rhodiola grandiflora in plateau, using test tube cultured seedlings as seedling source, the steps are as follows:

[0031] a. After soaking the seeds for 12 hours, first soak them in ethanol for 25 seconds, and then soak them in HgCl for 25 minutes;

[0032] b, then rinse with sterile water for 3 times, then dry the water on the surface of the explant for use;

[0033] c. The aseptic material obtained is inoculated on the Ms medium under sterile conditions. After the seeds germinate, they are transferred to 1 / 2 Ms medium to simulate the wild environment, control the temperature difference between day and night, and control the light time at 10 hours to induce The formation of lignified roots, and then the bottle seedlings that form lignified roots are transplanted into the substrate through hardening;

[0034] d. The hardened seedlings are planted in the field 5 months after transplanting, and can be harvested in 23 months.

[0035] In step...

Embodiment 3

[0040] A seedling raising method of Rhodiola grandiflora in plateau, using test tube cultured seedlings as seedling source, the steps are as follows:

[0041] a. After soaking the seeds for 24 hours, first soak them in ethanol for 35 seconds, and then soak them in HgCl for 30 minutes;

[0042] b, then rinse with sterile water for 5 times, then dry the water on the surface of the explant for use;

[0043] c. The obtained sterile material is inoculated on Ms medium under sterile conditions, and after the seeds germinate, transfer to 1 / 2 Ms medium to simulate the wild environment, control the temperature difference between day and night, control the light time at 11 hours, and induce The formation of lignified roots, and then the bottle seedlings that form lignified roots are transplanted into the substrate through hardening;

[0044] d. The hardened seedlings are planted in the field 8 months after transplanting, and harvested in 26 months. The time of planting and harvesting v...

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Abstract

The invention discloses a seedling raising method of plateau rhodiola crenulata, comprising the following steps of: firstly, preparing a seedling source by the tube culture of seedling, soaking a seed for 12-24h, wherein the seed is soaked in ethanol for 30 plus / minus 5s firstly, and then soaked in HgCl for 25-30min; secondly, washing with sterile water 3-5 times, and sipping up water on the surface of an explant for later use; thirdly, under the sterile condition, inoculating on an Ms culture medium, transferring onto 1 / 2 Ms culture medium after seed germination, simulating a wild environment, controlling a day and night temperature difference, inducing formation of a lignified root, and transplanting seedling in a matrix from a bottle after seedling adaptation; fourthly, field planting in the field about 6 months after plantlet transplanting, and harvesting about 24 months. The invention is mainly suitable for a seedling raising method for planting the rhodiola crenulata in a plateau area such as Tibet and the like without adopting hormone treatment, which takes the seed as an explant material and induces the lignified root by regulating a temperature difference and illumination of a culture room and sterile culture, and has the advantages of rapid reproductive speed and good hereditary.

Description

technical field [0001] The invention relates to a method for raising seedlings of medicinal plants, in particular to a method for raising seedlings of Rhodiola grandiflora. Background technique [0002] Rhodiola grandis is a perennial herb or subshrub of the genus Rhodiola in the Sedum family. It grows in the snow-capped mountains at an altitude of 3500-5000m on the Tibetan plateau, and is affected by harsh environments such as long-term hypoxia, dryness and strong ultraviolet rays, forming unique nutrients, rich in salidroside, flavonoids, multivitamins and trace elements, and Contains 17 kinds of amino acids necessary for the body. Studies in recent decades have shown that Rhodiola has many functions such as anti-hypoxia, anti-cold, anti-fatigue, anti-radiation, anti-virus, delaying the aging of the body, preventing senile diseases, etc., and has high medicinal value. Used as medicine, the immune effect is stronger than that of ginseng and eleuthero without the excessive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李宝海白玛玉珍李顺凯
Owner TIBET ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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