A kind of rapid propagation method of Yunnan laurel Ainaxiang medicinal material

A kind of fragrant medicinal material, fast technology, applied in the field of rapid propagation and cultivation of Yunnan Guilinaxiang medicinal material, can solve the problem that the survival rate, sterilization rate and induction rate of Yunnangui Ainaxiang medicinal material are not very high, and cannot be industrialized Production demand and other issues, to achieve the effect of low transportation cost, short cycle and low cost

Active Publication Date: 2016-05-18
广西桂西制药有限公司
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  • Application Information

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Problems solved by technology

Wei Pengxiao, Zhang Mingzhen and others conducted experimental research on the sterilization and callus induction of the medicinal material Ainaxiang yunnanensis and the tissue culture system of the medicinal material Ainaxiang yunnanensis. These contents are only the rapid propagation process of the medicinal material The laboratory research work of the basic theory is still different from the large-scale actual production in factories. Although it has very important reference value for the seedling cultivation technology of the medicinal material of Yunnan Guilin, it has not solved the low survival rate of the medicinal material of Yunnan Gui. The problem of high cost. At the same time, the effect of different sterilizing agents and different sterilization times on the sterilization effect of different explants was studied in the disinfection method of the explants in the tissue culture process of the medicinal material of Yunnan Gui Ainaxiang. This method has been verified by the laboratory. , it is indeed effective to disinfect the explants of Ainaxiang medicinal material, but the sterilization rate and induction rate are not very high, and the survival rate is low, which cannot meet the needs of factory production; in Wei Pengxiao, Zhang Mingzhen et al. The research on the tissue culture of Ainaxiang medicinal material also involves the influence of different phytohormones on the callus induction of Ainaxiang medicinal material. More important in factory farming

Method used

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  • A kind of rapid propagation method of Yunnan laurel Ainaxiang medicinal material
  • A kind of rapid propagation method of Yunnan laurel Ainaxiang medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Sterilization of the explants of the medicinal material explants

[0024] Cut the stem tip about 2cm long from the explant of the medicinal material of Yunnan Gui Ainaxiang, take 2~3ml of detergent, dissolve it in 2~3L of tap water, place the explant in the detergent water (the concentration is about 1‰) for 2 minutes, and shake constantly, then rinse with sterile water for 2~3 hours, and dry the surface with sterile filter paper. After pretreatment, the explant material was placed on an ultra-clean workbench and washed with 70% ethanol and 0.1% HgCl 2 The explants were sterilized and disinfected by the solution, rinsed with sterile water 5 times after each ethanol disinfection, and HgCl 2 Rinse 5 times with sterile water after treatment.

[0025]A total of 12 treatments were set up in this experiment, and each treatment was repeated 3 times. Disinfection method 70% ethanol disinfection treatment for 30s, 35s, 40s; 0.1%HgCl 2 Disinfection treatment for 3m...

Embodiment 2

[0029] Example 2: Induction, Differentiation, Proliferation and Rooting of Callus of Ainaxiang from Yunnan

[0030] ①Introduce the sterilized explants into the induction medium, the formula of the induction medium is MS basic medium, the concentration of BA is 3mg / L, the concentration of NAA is 0.2mg / L, and the concentration of superphosphate is 0.5gmg / L , add 25g / L sucrose as a carbon source, add 6g / L agar as a coagulant; adjust the pH value to 5.6 with 1mol / LNaOH and 1mol / LHCl; (where MS medium is a general basal medium, BA and NAA plant hormones , chemical reagents such as superphosphate, agar and acid-base solution are available in the market, and the present invention selects China Pharmaceutical (Group) Shanghai Chemical Reagent Company to produce, and the culture condition is dark culture, and after 20 days, the explant induces callus tissue, and the callus The induction rate of tissue can reach up to 95%;

[0031] ② Differentiation of callus: when the callus grows to ...

Embodiment 3

[0035] Example 3: Transplanting of Tissue Culture Seedlings of Diangui Ainaxiang Medicinal Material

[0036] Transplanting the tissue-cultured seedlings of Ainaxiang yunnanensis is a key link in the establishment of the rapid propagation system of Ainaxiang yunnanensis. The survival rate and growth of the tissue-cultured seedlings after transplanting are directly related to the rapid propagation of Ainaxiang yunnanensis. To ensure the success or failure of the propagation system, the transplanted seedlings should be tissue-cultured seedlings with well-developed root systems, strong growth, and no pests and diseases.

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Abstract

The invention discloses a rapid propagation method of a Blumea riparia (Bl.) DC medicinal material. The rapid propagation method comprises the steps: sterilizing an explant of the Blumea riparia (Bl.) DC medicinal material, inducing calluses, differentiating the calluses, proliferating multiple shoots, performing rooting culture on the multiple shoots, and transplanting tissue culture seedlings. The rapid propagation method of the Blumea riparia (Bl.) DC medicinal material disclosed by the invention is suitable for breeding and planting in a factorized scale, and has the advantages of low investment, high seedling culture speed, high survival rate, short period, consistent growth vigor of seedlings and the like, the raw material of the explant is easy to obtain, large in quantity and wide in range, the survival rate of tissue culture reaches 95%, moreover, the hereditary character is stable, the produced herbal material has stable quality, meanwhile, the Blumea riparia (Bl.) DC medicinal material is guaranteed to be stable in source, and the rapid propagation method provides a rapid and effective path to fully exert the ecological benefits and economic benefits of the Blumea riparia (Bl.) DC medicinal material in the southwest region of China.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation, and specifically relates to a method specially used for the rapid propagation and cultivation of yunnangui ainaxiang medicinal materials. Background technique [0002] Diangui'ainaxiang HERBABLUMEA ERIPARIAE is a plant of the genus Ainaxiang in the family Asteraceae, also known as Huaainaxiang and Guanya. It is a Chinese herbal medicine that grows in minority areas in Southwest China. It is the main product of Guangxi Guixi Pharmaceutical Co., Ltd. The main raw material of Fuxuekang Granules, the whole herb is used as medicine. The product has the functions of promoting blood circulation, removing blood stasis, hemostasis and regulating menstruation. The efficiency is more than 96%, and it is a plant with high economic value. [0003] Diangui Ainaxiang has strict requirements on the growth environment. It is suitable for growth in subtropical regions with temperatu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 包国庆黄河
Owner 广西桂西制药有限公司
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