PON-1 (paraoxonase-1) activity detection kit and detection method for same

Abstract

The invention discloses a PON-1 (paraoxonase-1) activity detection kit and a detection method for the same. The kit comprises two reagents, namely, a buffer solution and a substrate solution, wherein the substrate solution contains 0.15-0.42 mol / L 2-chloro-p-nitrophenyl diethyl phosphate and a solvent, namely, dimethyl sulfoxide; and the buffer solution contains 0.15 mol / L glycine, 2 mol / L NaOH, 0.0025 mol / L CaCl2 and an aqueous solvent, and has a pH value of 10.0-11.0. The detection method adopting the kit is rapid, simple and convenient, and early cardiovascular diseases can be effectively by measuring the activity of PON-1 in serum; and compared with the prior art, a synthesis method for 2-chloro-p-nitrophenyl diethyl phosphate is rapid, simple and convenient, safe, and high in yield.

Description

technical field [0001] The invention relates to a PON-1 activity detection kit with 2-chloro-p-nitrophenyl diethyl phosphate as a substrate and a detection method thereof, which are suitable for quantitative determination of PON-1 in body fluids such as human serum by a biochemical analyzer in vitro Activity, used for auxiliary diagnosis. Background technique [0002] Cardiovascular diseases, also known as circulatory system diseases, mainly include: heart disease, hypotension, hypertension, hyperglycemia, stroke, myocardial infarction, thrombosis, arteriosclerosis, etc., and its pathological basis is atherosclerosis. The prevention and early diagnosis of cardiovascular disease is one of the focuses of medical research in recent years. [0003] At present, it is often clinically determined by measuring serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), lipoprotein a (Lp-a ), apolipopro...

AI Technical Summary

Problems solved by technology

However, there is an obvious shortcoming in this method: these index parameters can objectively reflect cardiovascular function only when the cardiovascular tissue is seriously damaged, and there is a possibility of misdiagnosis, while in early cardiovascular disease, these indicators cannot Timely reflection of subtle changes in cardiovascular function

However, since the color source released by paraoxon substrate hydrolysis is close to the color of serum, the measurement will affect the data, and the background needs to be deducted; on the other hand, in terms of measurement accuracy, sensitivity and application range, colorimetry is not as good as Ultraviolet spectrophotometry, which has a large error in the determination of low-activity PON-1, needs to increase the volume of serum samples to meet the detection requirements for sensitivity

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