Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation

A technology for the harmless treatment of agricultural waste, applied in the field of microbial pesticides and environmental protection, can solve environmental pollution and other problems, achieve the effects of reducing trigger factors, improving soil, and reducing pollution

Inactive Publication Date: 2013-11-27
GRAPE & WINE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the means to prevent the above problems is mainly through incineration, but this method will not only cause environmental pollution, but also fail to realize the comprehensive recycling of this huge amount of biomass resources, so it cannot be regarded as an ideal treatment method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A technology for the harmless treatment of agricultural waste produced by viticulture, comprising the following steps:

[0023] (1) Seed culture: Dilute the molasses alcohol waste liquid to 6 ° Bx, add (NH 4 ) 2 SO 4 After being sterilized at 110°C for 30 minutes, it was used as a seed medium, and after cooling, the spore concentration of 4% of the volume fraction of the seed medium was inoculated at 10 6 spores / mL, the spore suspension of Trichoderma species, at 25°C, shake the flask at a shaking speed of 140r / min, ferment for 24h to prepare seed culture; Trichoderma species is Trichoderma viride;

[0024] (2) Liquid expansion culture: Dilute the molasses alcohol waste liquid to 6 ° Bx, add (NH 4 ) 2 SO 4 , after being sterilized at 110°C for 15 minutes, it was used as a liquid expansion medium, and 4% of the volume fraction of the liquid expansion medium was inserted into the seed culture, at 25°C, with a mechanical stirring speed of 200 r / min, and an aeration ra...

Embodiment 2

[0028] A technology for the harmless treatment of agricultural waste produced by viticulture, comprising the following steps:

[0029] (1) Seed culture: Dilute the molasses alcohol waste liquid to 7 ° Bx, add (NH 4 ) 2 SO 4After being sterilized at 115°C for 20 minutes, it was used as a seed medium, and after cooling, the spore concentration was 10% inoculated according to the volume fraction of the seed medium. 7 spores / mL, the spore suspension of Trichoderma species, at 30°C, shake the flask at a shaking speed of 180r / min, and ferment for 36h to prepare seed culture; Trichoderma species is Trichoderma viride;

[0030] (2) Liquid expansion culture: Dilute the molasses alcohol waste liquid to 7 ° Bx, add (NH 4 ) 2 SO 4 , after being sterilized at 115°C for 20 minutes, it was used as a liquid expansion medium, and 6% of the volume fraction of the liquid expansion medium was inserted into the seed culture, at 30°C, with a mechanical stirring speed of 300 r / min, and an aerat...

Embodiment 3

[0035] A technology for the harmless treatment of agricultural waste produced by viticulture, comprising the following steps:

[0036] (1) Seed cultivation: Dilute the molasses alcohol waste liquid to 8 ° Bx, add (NH 4 ) 2 SO 4 After being sterilized at 121°C for 15 minutes, it was used as a seed medium, and after cooling, the concentration of spores inoculated at 10% of the volume fraction of the seed medium was 10 8 spores / mL, Trichoderma species spore suspension, at 35°C, shake the flask at a shaking speed of 200r / min, ferment for 48h to prepare seed culture; Trichoderma species is Trichoderma harzianum;

[0037] (2) Liquid expansion culture: Dilute the molasses alcohol waste liquid to 8 ° Bx, add (NH 4 ) 2 SO 4 After being sterilized at 121°C for 15 minutes, it was used as a liquid expansion medium, and 10% of the volume fraction of the liquid expansion medium was inserted into the seed culture. At 35°C, the mechanical stirring speed was 400 r / min, and the aeration ra...

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PUM

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Abstract

The invention discloses a microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation. The method comprises steps of seed culture, liquid state fermentation and solid state fermentation. A Trichoderma strain is Trichoderma viride or Trichoderma harzianum. The invention not only provides a novel approach for harmlessness treatment and resource utilization of molasses alcohol wastes from sugarcane processing and a large amount of agricultural wastes generated by grape cultivation, besides, a solid Trichoderma agent obtained from the treatment can be applied to vineyards to increase biomass of Trichoderma for biological control in the environment, improve microecology and soil of the vineyard, inhibit pathogenic microorganisms for grapes in the microecology of the vineyard and reduce initiation factors for microbial diseases of grapes, thereby reducing application amount of pesticide, and enhancing fruit quality and security of the grapes.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and environmental protection, and in particular relates to a technology for the harmless treatment of agricultural waste produced by grape cultivation by a microbial method. Background technique [0002] Trichoderma ( Trichoderma sp. ), belonging to the Phylum Fungi, Deuteromycotina, Hyphospora, Hyphospora, Synchophyceae, and Trichoderma. It is widely present in soil under different environmental conditions and is an important biocontrol fungus. According to Its morphological characteristics are classified as: Trichoderma harzianum ( Trichoderma harziamum ), Trichoderma viride ( Trichoderma viride ), Trichoderma hacinata ( Trichoderma hamatum ), Trichoderma longibrachiata ( Trichoderma longibratum ), Trichoderma chrysogreen ( Trichoderma aureoviride )wait. Most Trichoderma can produce a variety of biologically active substances that have antagonistic effects on plant pathog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00
CPCY02W30/40
Inventor 李玮张瑛陈国品曹慕明李洪艳张劲周咏梅刘淑仪韩佳宇
Owner GRAPE & WINE RES INST GUANGXI ACADEMY OF AGRI SCI
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