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Application of 3,4-Dihydroxyphenylacetic acid in preparing antitumor drug

A technology of high protocatechuic acid and tumor drugs, which is applied in the field of high protocatechuic acid in the preparation of antitumor drugs, and can solve problems such as unclear toxic components

Inactive Publication Date: 2015-06-10
JINGPENGHUI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its specific toxic components are not clear, and the relationship between the toxic components and active components of Xanthophylla remains to be further studied

Method used

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  • Application of 3,4-Dihydroxyphenylacetic acid in preparing antitumor drug
  • Application of 3,4-Dihydroxyphenylacetic acid in preparing antitumor drug
  • Application of 3,4-Dihydroxyphenylacetic acid in preparing antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The effect of plateau catechin on the proliferation of tumor cells and normal cells

[0035] Experimental protocol: Using thiazole blue (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, MTT) experiment, plateau catechin (DO) (dose gradient is 10mg / ml, 1mg / ml, 100μg / ml, 10μg / ml, 1μg / ml, 100ng / ml) respectively treat lung cancer cell A549, lung cancer cell LLC, Wilms tumor cell G401, nasopharyngeal carcinoma cell CNE2Z and laryngeal carcinoma cell Hep2, rhabdomyosarcoma cell A204, breast cancer cell MDA231, breast cancer cell T47D, breast cancer cell MCF7, pancreatic cancer cell PANC1, neuroblastoma cell SY5Y and cervical cancer cell Hela for 24 hours, MTT staining for 4 hours, dimethyl sulfoxide (DMSO) dissolve, measure the absorbance value at 570nm with a microplate reader, take the compound gradient as the abscissa and the cell survival rate as the ordinate, draw a standard curve, and calculate the half inhibitory concentration IC 50 , The specific res...

Embodiment 2

[0039] Example 2 Anti-tumor cell migration effect of plateau catechin in vitro

[0040] Experimental program:

[0041] 1) Using cell migration detection (Transwell) experiment, 20μg / ml plateau catechin (DO) (safe dose) is used to treat lung cancer cells LLC, lung cancer cells (A549), cervical cancer cells Hela, neuroblastoma cells SY5Y, breast cancer Cell MCF7, breast cancer cell MDA231, breast cancer cell T47D, rhabdomyosarcoma cell A204, Wilms tumor cell G401, skin squamous cell carcinoma A431, nasopharyngeal carcinoma cell CNE2Z and laryngeal carcinoma cell Hep 224 hours later, fixed with anhydrous ice methanol 20 Minutes, and then stained with crystal violet for 15 minutes. Take pictures with a 100x light microscope to detect the number of cells migrated.

[0042] 2) Using Scratch Analysis experiment, 20μg / ml plateau catechin (DO) (safe dose) is used to treat lung cancer cells (LLC), lung cancer cells (A549), cervical cancer cells (Hela), neuroblastoma Cells (SY5Y), breast canc...

Embodiment 3

[0047] Example 3 The effect of plateau catechin on tumor cell apoptosis

[0048] Experimental protocol: Apoptosis flow cytometry experiment was used to treat human lung cancer cells (A549), Wilms tumor cells (G401) and laryngeal cancer cells (Hep2) with 10μM plateau catechin (DO) for 24 hours, and then centrifuge to collect Cells (1500rpm, 5min) were resuspended in PBS and centrifuged twice, and then stained with Annexin-V FITC and PI for 15min. Flow cytometry was used to detect the percentage of apoptotic cells, the sum of the percentages of early and late apoptotic cells And the total ratio of apoptosis.

[0049] Experimental results: High altitude catechin (DO) can significantly promote the apoptosis of A549, G401 and Hep2 cells, suggesting that high altitude catechin (DO) may play an anti-tumor effect by mediating tumor cell apoptosis. See the specific results image 3 A-3C.

[0050] The above in vitro anti-tumor effect experimental results indicate that plateau catechin can af...

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Abstract

The present invention provides applications of 3,4-Dihydroxyphenylacetic acid (DOPAC) or medically acceptable salt, solvolyte, or polycrystal thereof in preparing drugs for preventing and / or curing tumors. Experiments in vivo and vitro indicate that DOPAC can suppress multiple types of tumors such as lung cancer, breast cancer, cervical carcinoma, nephroblastoma, nasopharyngeal carcinoma, laryngeal carcinoma, and neuroblastoma, and has broad-spectrum antitumor functions. This type of compounds are derived from natural drugs, and hopefully provide new choices for clinical treatment.

Description

Technical field [0001] The invention belongs to the field of antitumor drugs, and specifically relates to the use of plateau catechins in the preparation of antitumor drugs. Background technique [0002] Tumors are new organisms formed by the body under the action of various carcinogenic factors, cells lose their normal regulation of their growth at the gene level, leading to their clonal abnormal proliferation, which manifests as masses. Tumor cells are different from normal cells in terms of external morphology, metabolism and function, and often exhibit continuous proliferation. According to effective statistics, tumors are currently the first of the three major factors threatening human life and health in the world, so the research and development of anti-tumor drugs has become a hot spot in recent years. [0003] According to the traditional classification and research progress of anticancer drugs, commonly used anticancer drugs can be divided into the following categories: 1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/192A61P35/00
CPCA61K31/192A61P35/00
Inventor 李梢
Owner JINGPENGHUI PHARMA
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