Gene encoding h5n1 subtype avian influenza synonymous hemagglutinin (ha) protein and synonymous neuraminidase (na) protein and application thereof

Abstract

The invention provides a recombinant expression vector for simultaneously expressing H5N1 subtype avian influenza virus synonymous hemagglutinin protein and synonymous neuraminidase protein, wherein the amino acid sequence of the synonymous hemagglutinin protein is SEQ ID NO:1, and the amino acid sequence of the synonymous neuraminidase protein is SEQ ID NO:2; the recombinant expression vector is formed through inserting the nucleotide sequence coded by SEQ ID NO:1 and the nucleotide sequence coded by SEQ ID NO:2 into the same expression vector. The invention provides a DNA vaccine including the recombinant expression vectors in effective quantity, which can be used for effectively preventing and / or treating infection of H5 subtype avian influenza virus; simultaneously, the invention further relates to the application of the protein or the coded amino acid sequence thereof serving as the inactivated vaccine for H5 subtype avian influenza virus and immunogen genes of other genetic engineering vaccines.

Description

technical field [0001] The present invention belongs to the field of molecular biology. Specifically, the present invention provides a recombinant expression vector that simultaneously expresses a synonymous hemagglutinin protein and a synonymous neuraminidase protein of the H5N1 subtype avian influenza, a DNA vaccine comprising an effective amount of the above-mentioned recombinant expression vector, and the vaccine It can effectively prevent and / or treat H5 subtype avian influenza virus infection. Background technique [0002] Avian influenza (Avian influenza virus, AIV) is a poultry severe infectious disease caused by type A influenza virus, which has spread all over the world so far, and H5 subtype highly pathogenic avian influenza (HPAI) is known for its high morbidity and high mortality rate, posing a major threat to the poultry industry. So far, many cases of human infection with H5N1 subtype avian influenza have been reported, and the mortality rate can be as high ...

AI Technical Summary

Problems solved by technology

The production of this vaccine relies on a large number of chicken embryos, and it is difficult to respond quickly according to changes in the epidemic situation when new strains appear, and the cross-protection between different strains is poor, which makes the vaccine strains have to Frequently updated (Subbarao and Joseph, 2007), some regions even need to use multiple vaccines targeting different antigen groups at the same time, which undoubtedly increases the difficulty of preventing and controlling the avian influenza epidemic

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