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Method for determining lotus pollen viability

A technology of lotus pollen and vitality, applied in the direction of plant cells, etc., to achieve the effect of simple detection method, scientific and reasonable determination method, and high practical application value

Inactive Publication Date: 2015-03-04
ZHENJIANG SUIHAN AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are no reports about the culture medium and culture conditions for in vitro germination of lotus pollen. The announcement of the present invention can provide the most suitable pollen in vitro germination culture system for lotus varieties, and provide an efficient, fast and simple method for measuring pollen vigor. Methods

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  • Method for determining lotus pollen viability

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Embodiment

[0016] The present embodiment measures the vigor of lotus pollen by the following methods:

[0017] Step 1, adopt distilled water to prepare liquid culture medium, described liquid culture medium contains 250g / L sucrose, 10mg / L H 3 BO 3 , ME3 medium (370mg / L MgSO 4 7H2O, 950mg / L KNO 3 , 880mg / L CaCl 2 2H 2 O, 0.025mg / L FeSO 4 ·7H 2 O and 0.83mg / L KI), 100g / L PEG-1500;

[0018] Step 2: Collect pollen. In late July, when the lotus variety 'Youyihong' (which can be purchased from Nanjing Yilianyuan Flower Co., Ltd.) blooms, before collecting pollen, the collected inflorescences are artificially bagged and isolated the day before flowering. Collect blooming lotus pollen around 8:00 in the morning, put the collected pollen into a centrifuge tube, and store it away from light;

[0019] Step 3: Cultivate pollen, drop 3 drops of liquid medium on the slide, dip the pollen into the liquid medium with a brush, directly place the slide in a petri dish lined with moist filter paper...

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Abstract

The invention relates to a method for determining pollen viability, and particularly relates to a method for determining lotus pollen viability, belonging to the technical field of plants. The invention discloses a method for determining lotus variety pollen viability by using an in-vitro germination method, which is capable of effectively determining the lotus pollen viability. A pollen in-vitro germination liquid culture medium used in the method uses distilled water as a solvent and comprises the following components of 250g / L saccharose, 10mg / L H3BO3, partial ME3 and 100g / L PEG1500 (polyethylene glycol). The method comprises the steps of dropping a culture solution on a glass slide, dipping a little pollen in a culture medium by using a writing brush without being covered by a cover glass, placing the glass slide in a culture dish cushioned with wet filter paper for being cultured at a temperature of 25 DEG C in a dark condition after sowing; and observing the germination of the pollen and the growth condition of a pollen tube by using an optical microscope after 4h. The method is simple and completely quantitative, reliable in data, strong in maneuverability and higher in actual application values, and is of great importance in increasing the breeding efficiency of lotus.

Description

technical field [0001] The invention relates to a method for measuring pollen vigor, in particular to a method for measuring lotus pollen vigor, and belongs to the technical field of plants. Background technique [0002] Lotus (Nelumbo nucifera) is a perennial emergent flower of the genus Nymphaeaceae. my country has a splendid lotus culture and a long history of cultivation. As one of the top ten famous flowers in my country, the lotus has various varieties, unique flower shapes, and far-reaching fragrance. It blooms in the hot summer and is the main aquatic plant for waterscape in Chinese gardens. At present, pollen germination requires certain conditions: moisture, temperature, sugar, pH value, mineral elements, vitamins, etc., among which sugar and mineral elements are the dominant factors. Sucrose can not only adjust the osmotic potential of pollen so that it will not burst due to excessive water absorption, but also can be used as a respiratory substrate and a raw mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 孙春青潘跃平戴忠良马志虎孙国胜
Owner ZHENJIANG SUIHAN AGRI
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