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Ovomucin composite solubilizer and solubilization method of ovomucin

A technology of ovomucin and solubilizer, which is applied in the field of ovomucin compound solubilizer, can solve the problems of low solubility of ovomucin, achieve the effects of improving solubility, protecting natural conformation, and promoting adhesion

Active Publication Date: 2013-12-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound solubilizing agent for ovomucin and a method for solubilizing ovomucin in order to overcome the defect of low solubility of ovomucin

Method used

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  • Ovomucin composite solubilizer and solubilization method of ovomucin
  • Ovomucin composite solubilizer and solubilization method of ovomucin
  • Ovomucin composite solubilizer and solubilization method of ovomucin

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Take 1g of ovomucin and 2.4g of ovomucin complex solubilizer (containing 2.0g of arginine and 0.4g of glycerol), add it to 100mL of water (the concentration of arginine in the solution is 115mmol / L), and mix well after constant volume , stirred for 6-8 hours, centrifuged at 1500r / min for 5 minutes, and the nitrogen content in the added total protein, supernatant and corresponding solvents were measured by the Kjeldahl method, and the solubility of the solvent was expressed by nitrogen solubility index.

[0018]

[0019] The determination of the solubility of ovomucin was carried out with reference to the methods known in the existing literature (Hiidenhovi et al, 2008), according to A 280 =KCL, where K is the absorbance coefficient, calculated according to the average coefficient of protein 1L / (g cm), then the concentration of ovomucin C(mg / mL)=A 280 .

[0020] The nitrogen solubility index and solubility measured by this embodiment are shown in Table 2.

Embodiment 2～6

[0022] In Examples 2 to 6, the dosage and formula of the ovomucin complex solubilizer are shown in Table 1. The steps of the ovomucin solubilization method are the same as in Example 1, that is, ovomucin and the ovomucin compound solubilizer are used. , added to 100mL of water, mixed evenly after constant volume, stirred for 6-8h, and centrifuged at 1500r / min for 5min.

[0023] Table 1 The dosage and formula of ovomucin complex solubilizer in Examples 1-6

[0024]

[0025] Table 2 shows the results of the solubility of ovomucin in arginine and its salt solution determined by this embodiment.

[0026] Table 2 Solubility of ovomucin in Examples 1-6

[0027]

[0028] It can be seen from the above tests that the solubility of ovomucin without adding a solubilizing agent is very poor. By the method of the present invention, the nitrogen solubility index of ovomucin is increased from about 2% to more than 15%, and the solubility is increased by more than 6 times.

Embodiment 7

[0029] Example 7 The adhesion ability of ovomucin solution to Newcastle disease virus

[0030] The adhesion performance of the ovomucin solution prepared in Examples 1-6 to Newcastle Disease Virus (NDV) was determined by enzyme-linked immunosorbent assay (ELISA). The ovomucin solution of Examples 1-6 was dissolved in 50 mol / L borate buffer (pH 9.6), and the protein concentration was adjusted to 100 μg / mL. Add 100 μL of ovomucin to each well of the 96-well ELISA plate, fix overnight at 4°C, gently aspirate the supernatant, and wash 1-2 times with PBS-T to remove unfixed protein. Add 100 μL BSA to each well, incubate at 37°C for 2 hours to shield non-specific binding sites, and then wash with PBS-T 1-2 times. After adding 200 μL of NDV (4 hemagglutination units) to the experimental wells, incubate at 37°C for 60 min, gently suck off excess virus liquid, and then wash with PBS 1-2 times to remove non-adhered virus. Add 100 μL of NDV antibody, continue to incubate at 37°C for 1 ...

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Abstract

The invention discloses an ovomucin composite solubilizer, comprising polar aliphatic amino acid or salt of polar aliphatic amino acid and polyhydric alcohols, wherein the weight ratio of the polar aliphatic amino acid or salt of polar aliphatic amino acid to the polyhydric alcohols is (5-10): 1; and the polar aliphatic amino acid is one or mixture of arginine and glutamic acid. The invention also discloses a solubilization method of ovomucin. According to the solubilization method, the ovomucin and the ovomucin composite solubilizer are dissolved into water to prepare solution, wherein the weight ratio of the ovomucin to the ovomucin composite solubilizer is 1:(2-8), so that the concentration of the polar aliphatic amino acid or salt of the polar aliphatic amino acid in the solution is 100-400 mmol / L. By adopting the solubilization method, the solubility of the ovomucin can be effectively improved; meanwhile, the antiviral effect of the ovomucin is enhanced; and the ovomucin composite solubilizer is applicable to the fields of foods and medicines.

Description

technical field [0001] The invention relates to a compound solubilizing agent for ovomucin and a method for solubilizing ovomucin. Background technique [0002] Ovomucin is one of the very important proteins in egg white. It has various biological activities such as antibacterial, antiviral, inhibiting the proliferation of tumor cells and lowering serum cholesterol. It has important research and development value in medicine, nutrition and health care. . However, the ovomucin prepared by the precipitation method in the prior art is usually a white, cohesive floc, which is insoluble in water and common salt solutions. The low solubility of ovomucin greatly affects and limits its The full play of many functional properties, so improving and enhancing the solubility has always been one of the important contents of ovomucin research. [0003] The current method to improve the solubility of ovomucin is mainly by adding denaturants or reducing agents, such as SDS, urea, guanidin...

Claims

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Application Information

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IPC IPC(8): C07K14/465
Inventor 马美湖单媛媛黄茜高菲
Owner HUAZHONG AGRI UNIV
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