Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof

A technology of o-xylene and kinetoplastus, which is applied in the field of biological treatment of environmental pollutants, can solve the undiscovered problems of degrading o-xylene, and achieve the effect of low purification cost, easy promotion, and high-efficiency purification

Active Publication Date: 2013-12-18
浙江泷赢环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieving patents and other relevant literature, no reports have been found on the use of this strain to degrade o-xylene

Method used

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  • Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof
  • Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof
  • Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one, Zoogloea resiniphila Isolation, purification and identification of HJ1.

[0026] 1. Zoogloea resiniphila Isolation and purification of HJ1.

[0027] Zoogloea resiniphila HJ1 is a Gram-negative bacterium domesticated and isolated from the activated sludge of the wastewater treatment station of Xinchang Pharmaceutical Factory. Specific steps are as follows:

[0028] Take the activated sludge from the wastewater treatment station of Xinchang Pharmaceutical Factory, wash it with tap water five times, and then air-expose it for 48 hours to remove the residual organic matter as much as possible. Inorganic salt culture solution (pH7) was prepared, and the activated sludge was directional domesticated with ortho-xylene as the only carbon source. Fresh inorganic salt culture solution was replaced every 2 days, and the pH of the culture solution was measured every day. Prepare an inorganic salt medium with an initial concentration of o-xylene of 100 mg / L,...

Embodiment 2

[0036] Embodiment two, Zoogloea resiniphila The degradation performance of HJ1 to different concentrations of o-xylene was tested.

[0037] Under the optimal environmental factor conditions (culture medium pH 7, culture temperature 35 ℃), the degradation performance of strain LX-1 to the initial concentration of 32-288mg / L o-xylene was investigated. The results showed that strain HJ1 could completely degrade 32-256 mg / L o-xylene, but only partially degrade the initial concentration of 288 mg / L o-xylene. Under the optimal culture conditions, the maximum specific degradation rate of the strain reached 0.19 mg o -xylene / (mg cells h). The specific implementation steps are as follows:

[0038] Prepare 1000 mL of inorganic salt culture solution with pH=7, distribute it in 250 mL saline bottles, 50 mL per bottle, and sterilize at 110 °C for 40 min. After the culture solution is cooled, take 10 bottles of it and add the HJ1 bacterial suspension in the logarithmic growth phase, a...

Embodiment 3

[0040] Embodiment three, Zoogloea resiniphila Mineralization rate and average yield coefficient analysis of o-xylene by HJ1.

[0041] Under the conditions of optimal environmental factors (culture medium pH 7, culture temperature 35°C), the strains were investigated Zoogloea resiniphila Mineralization rate and average yield coefficient of HJ1 for initial o-xylene concentrations of 32-258 mg / L. The results show that the strain HJ1 can finally convert o-xylene into CO 2 、H 2 O and cell biomass with an average mineralization rate of 54.8% and an average yield coefficient of 0.2572 mg cells / mg o -xylene. The specific implementation steps are as follows:

[0042] Inoculate the bacterial suspension in 32, 64, 92, 128, 160, 192, 224, 256 and 288 mg / L degradation medium with o-xylene as the only carbon source, seal it, and store it at 35°C and pH=7.5 under cultivation. Determination of the initial bacterial concentration and CO in the culture flask 2 concentration. Then, a...

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Abstract

The invention relates to Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof in an ortho-xylene organic pollutant. The collection number of the Zoogloearesiniphila HJ1 is CCTCC NO. M2012235. The invention also provides application of the Zoogloearesiniphila HJ1 with the ortho-xylene degradation capacity in degrading organic pollutants, namely benzene, methylbenzene, ethylbenzene, dimethylbenzene and chlorobenzene. The Zoogloearesiniphila HJ1 disclosed by the invention can be used for efficiently degrading the benzene series pollutants, such as ortho-xylene, and is high in tolerance capacity.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a kinetoglobium with the ability to degrade o-xylene ( Zoogloea resiniphila ) HJ1 and its applications. Background technique [0002] o-xylene (C 8 h 10 , O -xylene) is a chemical raw material for the production of phthalic anhydride (phthalic anhydride, PA), dyes, pesticides, etc., of which about 90% is used for the production of phthalic anhydride. Its vapor and air form an explosive mixture, which can cause combustion and explosion in case of open flame and high heat. Its vapor is heavier than air, and can spread to a relatively long distance at a lower place, and it will burn back when it encounters a fire source. In case of high heat, the internal pressure of the container will increase, and there is a danger of cracking and explosion. If the flow rate is too fast, it is easy to generate and accumulate static electri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34C12R1/01C02F101/32
CPCY02A50/20Y02P20/59
Inventor 於建明成卓韦蒋轶锋朱润晔顾信娜
Owner 浙江泷赢环境科技有限公司
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