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Method for increasing panicle length of rice

A technology for rice and ear length, applied in the fields of agriculture and molecular biology, and can solve undisclosed problems

Inactive Publication Date: 2013-12-18
HENAN NORMAL UNIV
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  • Abstract
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Problems solved by technology

The prior art has disclosed a rice Rho protein regulatory gene OsRhoGDI2 (Liang Weihong, Tang Chaorong, Wu Naihu. Separation and characteristic analysis of two kinds of rice GDP dissociation inhibitory protein genes, Chinese Journal of Biochemistry and Molecular Biology, 2004, 20 (6): 785-791), and registered on GenBank, the accession number is AY364312, but it is not disclosed that this gene is the key gene controlling the panicle length traits of rice

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  • Method for increasing panicle length of rice
  • Method for increasing panicle length of rice

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Embodiment 1

[0012] 1. Construction of rice OsRhoGDI2 gene overexpression vector

[0013] Design primers P1: (5'-AACTGCAGGAACCCTCCTCCTCCTCC-3', containing PstI restriction site) and P2: (5'-AACCTAGGGGACTTGCAGGGCCAGTC-3', containing AvrII restriction site), amplified by PCR into the cDNA coding region of the OsRhoGDI2 gene PstI and AvrII restriction sites were added at the 5' and 3' ends, respectively, and were connected to the corresponding sites of the plant expression vector pCAMBIA1302 (purchased from CAMBIA). The ligation product was transformed into Escherichia coli DH5α, and the transformants were screened on LB plates containing kanamycin. After digestion and PCR identification, sequencing was confirmed (the molecular weight of the pCAMBIA1302-OsRhoGDI2 vector was 11344 bp).

[0014] 2. Genetic transformation of rice, screening and analysis of rice traits of T2 generation transgenic OsRhoGDI2 gene

[0015] Using conventional rice genetic transformation methods, the OsRhoGDI2 gene o...

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Abstract

The invention provides a method for increasing the panicle length of rice. The method comprises the following steps: transfecting an expression vector containing OsRhoGDI2 genes into the rice, and then cultivating the rice to obtain transgenic rice containing overexpression OsRhoGDI2 genes of T2 generation. Due to the adoption of the scheme, the transgenic rice containing overexpression OsRhoGDI2 genes of T2 generation is obtained; compared with a control group, the transgenic rice has the characteristic that the panicle length is increased by 21.3-32.9 percent.

Description

technical field [0001] The invention belongs to the fields of agriculture and molecular biology, and in particular relates to a method for improving the ear length of rice through genetic engineering technology. Background technique [0002] Rice is one of the most important food crops, providing food and nutrition for more than half of the world's population. The improvement of rice varieties characterized by dwarf stalk breeding and utilization of heterosis has made two leaps in rice yield and made great contributions to solving the food problem in my country. However, in recent years, traditional rice breeding is facing the situation that it is difficult to improve the yield potential. At the same time, with the continuous increase of my country's population and the gradual reduction of arable land, food security has become a prominent problem. The improvement and improvement of rice yield and quality has always been the core issue of variety improvement. [0003] In re...

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H4/00
Inventor 梁卫红尚飞彭威风李莉谢先芝杨献光黄俊骏王华华
Owner HENAN NORMAL UNIV