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Method for cultivating eucalyptus tissue culture seedling by adopting light media container

A cultivation method and a light-substrate technology are applied in the cultivation field of excellent eucalyptus clone seedlings, which can solve the problems of low survival rate and emergence rate of bottle seedlings transplanted, great influence on the growth of tissue-cultured seedlings, and improper seedling management measures, etc., to achieve Light weight, short seedling period, and reduced labor intensity

Inactive Publication Date: 2014-01-01
沈云
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AI Technical Summary

Problems solved by technology

However, the ratio of light substrate raw materials used by different nursery units is not the same, which has a great impact on the growth of tissue cultured seedlings. At the same time, due to improper management of seedlings, it is easy to cause low survival rate and nursery rate of bottle seedlings, and low incidence of seedlings. higher

Method used

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Embodiment Construction

[0020] The healthy semi-ligninized eucalyptus branches will be collected and washed with detergent, 70% alcohol soaked, 0.1% mercuric chloride solution disinfected on the day of collection, and washed with sterile water for 3 times; then inoculated with sterilized inoculation equipment , Cut the required explant culture part and move it into the induction medium to induce sterile shoots. Cut the induced aseptic buds again under aseptic conditions, put them into the subculture medium for propagation, make them form clump buds, and replicate regularly. The temperature in the culture room is controlled at 27℃, and the light time is 15 hours a day. ; Cut the robust secondary shoots and insert them into the rooting medium to induce the shoots to take root. The inoculated rooting seedlings are first cultivated in low light for about 7 days. After the root emergence rate of the incision reaches 80% or more, they are transferred to the greenhouse for natural light culture for 16-18 day...

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Abstract

A method for cultivating a eucalyptus tissue culture seedling by adopting a light media container is characterized by adopting plant tissue culture and rapid propagation, optimizing ratio and treating processes of light media raw materials, improving a rooting bottle seedling hardening method and selecting a small atomized insecticide spraying machine to replace a method for manually spraying insecticide. The light media container has good water permeability and air permeability and is light, so that the labor intensity is reduced; root systems are developed, nursery stocks are strong, and the seedling recovering period is short after afforestation, thus, costs for seedling culture and afforestation are reduced; and moreover, rooting bottle seedling yield is higher than 98%, transplanting survival rate of bottle seedlings is higher than 95%, seedling morbidity is lower than 2%, the seedling culture period is relatively shortened to 45-60 days, then, the outplanting can be performed, the outplanting rate of large seedlings is higher than 90%, and the survival rate of afforestation is increased by 15%.

Description

Technical field [0001] The invention relates to a method for cultivating superior clonal seedlings of eucalyptus, in particular to a method for cultivating eucalyptus seedlings in a light substrate container tissue culture. Background technique [0002] Eucalyptus is the general name of Eucaluptus plants in the Myrtacae family. It is known as one of the three fast-growing tree species in the world. The systematic introduction and cultivation of eucalyptus in China began in the 1980s as a Sino-Australian international cooperative research project At the beginning, through the implementation of the project, the introduction and genetic improvement of eucalyptus in China has achieved remarkable results, and a number of excellent tree species and excellent clones have been selected, such as the excellent species E. grandis, Eucalyptus urophylla, and the DH32 series. Clones; At present, the improved fast-growing eucalyptus is the main afforestation tree species in South China and Sout...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCY02A40/22Y02P60/40
Inventor 沈云庞贞武吴兵梁秀莉黄全东苏勇唐再生陈德文莫继有兰俊龚卫新
Owner 沈云
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