Cartilage staining solution, bone staining method and method for preparing embryonic bone specimen by using bone staining method

Abstract

The invention discloses a cartilage staining solution, a bone staining method and a method for preparing an embryonic bone specimen by using the bone staining method. The pH range of the staining solution is 5-7; each liter of staining solution contains at least 0.14g of alcian blue. The cartilage staining solution has the beneficial effects that the bone can be quickly stained by using the alcian blue and alizarin red; the alcian blue and the alizarin red are acidic material dyes; the alcian blue is applied to dyeing of cartilage in an animal bone; the alizarin red is used for dyeing of hard bone in the bone; the cartilage staining solution is significant in dyeing effect, clear in color difference of the bone and muscle, simple in dyeing method, and free of a lot of manpower and material resources. In addition, the in vivo bone part is displayed by using the fabrication method of the duck embryo bone specimen under the condition of keeping a complete appearance of the specimen; observation is facilitated; convenience is supplied for research and teaching; the fabrication method is simple to operate, and short in period; the specimen can be fabricated by general scientific and technical personnel.

Description

technical field [0001] The invention relates to the field of specimen preparation, in particular to a cartilage staining solution, a bone staining method and a method for preparing embryonic bone specimens using the method. Background technique [0002] Poultry bones do not form the secondary ossification center of the epiphysis during growth and development, so there is no epiphysis and epiphyseal cartilage, and the lengthening of bones mainly depends on the proliferation and ossification of cartilage at the end of the bone. The bones of poultry are quite different from those of mammals. The bones of poultry have the characteristics of high strength and light weight, contain more inorganic calcium salts, and some bones in the trunk have been healed with each other. [0003] Animal specimens play a particularly important role in biological research, teaching, and popular science work. Skeleton specimens are the main materials for studying the morphology and structure of anim...

AI Technical Summary

Problems solved by technology

[0003] Animal specimens play a particularly important role in biological research, teaching, and popular science work. Skeleton specimens are the main materials for studying the morphology and structure of animal bones and their relationship with other organs. Animal bone specimens and bone specimen production techniques are also found in some Textbooks and magazine articles, the traditional bone specimen production methods generally include: fleshing, bone marrow removal, corrosion, bleaching, stringing and other steps, the production process is cumbersome, especially the fleshing and final stringing steps, the workload is large and requires manipulation Those who have strong professional knowledge and operation skills, otherwise it will cause bone damage and bone installation errors, and the production cycle will be long. In addition, the traditional bone production method is mainly suitable for relatively large animals and animals whose bone development is basically completed. , for embryos whose bones have not been calcified and hardened, it is difficult to complete the specimen preparation work, the practicability is low, and the scope of application is relatively limited. It is not suitable for smaller animals like domestic ducks, so it cannot be widely used by people.

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